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Title: Analysis Of Adaptive Response To DNA Damage Checkpoint Induced Arrest
Keywords: Cdc5 polo kinase, DNA damage checkpoint, adaptation, Ddc2, oscillation, transcription factors
Issue Date: 1-Sep-2009
Citation: YIO WEE KHENG (2009-09-01). Analysis Of Adaptive Response To DNA Damage Checkpoint Induced Arrest. ScholarBank@NUS Repository.
Abstract: In yeast Sacchromyces cerevisiae, a double strand break (DSB) leads to activation of DNA damage checkpoint. If the DSB is not repaired, after a prolonged G2/M arrest, it is eventually followed by an escape from the arrest -- a phenomenon known as adaptation. In the first part of this study, the role of Cdc5 polo kinase in the adaptive response was investigated. We have found that ectopic expression of Cdc5 accelerates the adaptation. In addition, we have shown that Cdc5 is necessary for extinguishing the checkpoint by inhibiting the recruitment of checkpoint response protein Ddc2. In the second part, we analyzed the gene expression response due to oscillatory transcription factors (TFs) mathematically. We have demonstrated that an oscillating TF could up-regulate a set of genes while others could be down-regulated or remain unchanged. Therefore, oscillatory behaviour provides the TFs with additional degree of dynamics and renders specificity to their responses.
Appears in Collections:Ph.D Theses (Open)

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