Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/23721
DC Field | Value | |
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dc.title | Proton Beam Fabricated Lab-on-a-chip for Single Molecule Detection | |
dc.contributor.author | CHAN SOOK FUN | |
dc.date.accessioned | 2011-06-30T18:01:01Z | |
dc.date.available | 2011-06-30T18:01:01Z | |
dc.date.issued | 2011-01-13 | |
dc.identifier.citation | CHAN SOOK FUN (2011-01-13). Proton Beam Fabricated Lab-on-a-chip for Single Molecule Detection. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/23721 | |
dc.description.abstract | Lab-on-a-chip (LOC) plays a significant role for the analysis of small volumes of biological entities. Proton beam writing (PBW) is a powerful direct-lithographic process with the ability to fabricate smooth high-aspect-walls in various polymers. For such LOC devices, a smooth microchannel improves the device's signal-to-noise-ratio by reducing the amount of scattered light. An SU-8 master mold was fabricated using PBW and the final LOC was produced using soft lithography in polydimethylsiloxane (PDMS).The device consists of a microfluidic channel, which has a dimension of 25µm (Width) by 40µm (Height) by 1cm (Length) and two aligned etched-multimode-optical-fibres embedded in PDMS. Fluorescence within the optical-fiber detection region is excited by a 488nm-laser, which was focused to the detection region by a microscope objective. The fluorescence emission from the detection region is monitored wavelength selectively by avalanche-photodiodes-detector. This LOC device is designed primarily for the purpose of single molecule detection in very low concentrations of nucleic acid tagged with quantum dots. | |
dc.language.iso | en | |
dc.subject | Proton beam writing (PBW), lab-on-a-chip (LOC), polydimethylsiloxane (PDMS), single molecule detection, microfluidic | |
dc.type | Thesis | |
dc.contributor.department | CHEMISTRY | |
dc.contributor.supervisor | WATT, FRANK | |
dc.contributor.supervisor | WOHLAND, THORSTEN | |
dc.contributor.supervisor | BETTIOL, ANDREW ANTHONY | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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ChanSF.pdf | 3.59 MB | Adobe PDF | OPEN | None | View/Download |
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