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Title: Proton Beam Fabricated Lab-on-a-chip for Single Molecule Detection
Keywords: Proton beam writing (PBW), lab-on-a-chip (LOC), polydimethylsiloxane (PDMS), single molecule detection, microfluidic
Issue Date: 13-Jan-2011
Citation: CHAN SOOK FUN (2011-01-13). Proton Beam Fabricated Lab-on-a-chip for Single Molecule Detection. ScholarBank@NUS Repository.
Abstract: Lab-on-a-chip (LOC) plays a significant role for the analysis of small volumes of biological entities. Proton beam writing (PBW) is a powerful direct-lithographic process with the ability to fabricate smooth high-aspect-walls in various polymers. For such LOC devices, a smooth microchannel improves the device's signal-to-noise-ratio by reducing the amount of scattered light. An SU-8 master mold was fabricated using PBW and the final LOC was produced using soft lithography in polydimethylsiloxane (PDMS).The device consists of a microfluidic channel, which has a dimension of 25µm (Width) by 40µm (Height) by 1cm (Length) and two aligned etched-multimode-optical-fibres embedded in PDMS. Fluorescence within the optical-fiber detection region is excited by a 488nm-laser, which was focused to the detection region by a microscope objective. The fluorescence emission from the detection region is monitored wavelength selectively by avalanche-photodiodes-detector. This LOC device is designed primarily for the purpose of single molecule detection in very low concentrations of nucleic acid tagged with quantum dots.
Appears in Collections:Master's Theses (Open)

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