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Title: Investigating the transcriptional regualtion of the stevor multi-gene family in plasmodium Falciparum
Keywords: Malaria, Plasmodium, transcription, stevor, regulation, gene
Issue Date: 22-Dec-2010
Citation: MADELEINE WYSS (2010-12-22). Investigating the transcriptional regualtion of the stevor multi-gene family in plasmodium Falciparum. ScholarBank@NUS Repository.
Abstract: The multi-gene family stevor in Plasmodium falciparum is thought to play a role in antigenic variation and virulence. Although transcriptional regulation in the related var multi-gene family has been shown to be mediated, in part, by promoter activity within its intron, the possible role of the stevor intron or the var intron in stevor¿s transcriptional regulation is not known. Transient transfection experiments using renilla and firefly luciferase reporter genes were performed to investigate the transcriptional regulation of stevor using constructs that shared a stevor gene with or without a downstream var intron. Unsuccessful transfections provided the basis for modifying the approach: constructs that had a lengthened upstream region from the stevor gene start site, with the intent of covering the complete promoter region of stevor, were cloned and used for subsequent transient transfections. Successful transfections were accomplished, and preliminary results based on one transfection suggest that the promoter of stevor is not affected by the var intron. Further experiments are required to confirm this finding. Due to limiting information about stevor transcriptional regulation, the determination of one basic characteristic of this multi-gene family was attempted, namely the lengths of the 5¿UTRs of this multi-gene family. Unfortunately, this was not able to be determined; however, with a few modifications in the protocol, future experiments will allow the elucidation of the lengths of these 5¿UTRs. Repeating these transfections and following the suggested modifications in the protocol of the 5¿UTRs experiments will allow us to come one step closer to understanding the transcriptional regulation of this important multi-gene family of Plasmodium falciparum.
Appears in Collections:Master's Theses (Open)

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