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https://scholarbank.nus.edu.sg/handle/10635/236748
Title: | ATRIAL NATRIURETIC PEPTIDE: A FOCUS ON TISSUE HETEROGENEITY AND TISSUE SPECIFIC FUNCTION | Authors: | ANG WEI FONG | ORCID iD: | orcid.org/0000-0002-2723-8946 | Keywords: | Atrial natriuretic peptide, tissue specific, tissue heterogeneity, arterioles, receptor isoforms, microperfusion | Issue Date: | 18-Aug-2022 | Citation: | ANG WEI FONG (2022-08-18). ATRIAL NATRIURETIC PEPTIDE: A FOCUS ON TISSUE HETEROGENEITY AND TISSUE SPECIFIC FUNCTION. ScholarBank@NUS Repository. | Abstract: | Human atrial natriuretic peptide (ANP) is a main regulator of our blood pressure/ volume homeostasis. It interacts with its receptor NPR-A and induces vasodilation, diuresis and natriuresis. Previously, our lab designed ANP analogues with exclusive diuretic (DGD-ANP) or vasodilatory (DRD-ANP) functions. To understand their tissue specific function, I evaluated the role of three natural NPR-A isoforms generated by alternative splicing. All three NPR-A isoforms failed to produce cGMP in mammalian cells but significantly reduced cGMP production of WT NPR-A. Thus, the differential expression of isoforms could play a role in tissue specific activity of ANP. In the kidney, ANP vasodilates afferent arterioles and vasoconstricts efferent arterioles to enhance diuresis. Although diuretic DGD-ANP shows similar effects as ANP, non-diuretic DRD-ANP has no such differential effects on renal arterioles. Thus, for the first time, I demonstrated the importance of signal transduction changes in renal arterioles for the distinct pharmacology of ANP analogues. | URI: | https://scholarbank.nus.edu.sg/handle/10635/236748 |
Appears in Collections: | Ph.D Theses (Open) |
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Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
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Afferent arteriole_ANG II + ANP_14.12.2021.mov | 25.8 MB | Video Quicktime | OPEN | None | Preview online | |
AngWF.pdf | 6.95 MB | Adobe PDF | OPEN | None | View/Download | |
Afferent arteriole_ANG II + cGMP_05.10.2022 IV.mov | 36.63 MB | Video Quicktime | OPEN | None | Preview online | |
Afferent arteriole_ANG II + DGD_03.01.2022.mov | 31.34 MB | Video Quicktime | OPEN | None | Preview online | |
Afferent arteriole_ANG II + DRD_11.05.2022.mov | 32.68 MB | Video Quicktime | OPEN | None | Preview online | |
Afferent arteriole_ANP alone_without ANG II_02.05.2022.mov | 31.67 MB | Video Quicktime | OPEN | None | Preview online | |
Afferent arteriole_cGMP alone_without ANG II_12.10.2022 IV.mov | 28.11 MB | Video Quicktime | OPEN | None | Preview online | |
Afferent arteriole_DGD alone_without ANG II_05.05.2022.mov | 37.8 MB | Video Quicktime | OPEN | None | Preview online | |
Afferent arteriole_DRD alone_without ANG II_11.05.2022.mov | 27.69 MB | Video Quicktime | OPEN | None | Preview online | |
Control_Afferent arteriole_with ANG II_19.05.2022.mov | 30.08 MB | Video Quicktime | OPEN | None | Preview online | |
Control_Afferent arteriole_without ANG II_18.03.2022.mov | 30.52 MB | Video Quicktime | OPEN | None | Preview online | |
Control_Efferent arteriole_with ANG II_13.12.2021.mov | 22.55 MB | Video Quicktime | OPEN | None | Preview online | |
Control_Efferent arteriole_without ANG II_22.03.2022.mov | 27.97 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_ANG II + ANP_17.11.2021.mov | 27.03 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_ANG II + cGMP_05.10.2022.mov | 34.03 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_ANG II + DGD_14.12.2021.mov | 31.69 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_ANG II + DRD_02.05.2022.mov | 35.27 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_ANP alone_without ANG II_07.12.2021.mov | 21.48 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_cGMP alone_without ANG II_11.10.2022 V.mov | 31.01 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_DGD alone_without ANG II_04.01.2022.mov | 29.27 MB | Video Quicktime | OPEN | None | Preview online | |
Efferent arteriole_DRD alone_without ANG II_09.05.2022.mov | 29.17 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_2nd Antibody only_individual_AF488, DAPI, WGA.mov | 2.47 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_2nd Antibody only_merge_AF488, DAPI, WGA.mov | 2.21 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_Isoform 1_individual_AF488, DAPI, WGA.mov | 2.32 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_Isoform 1_merge_AF488, DAPI, WGA.mov | 1.6 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_Isoform 2_individual_AF488, DAPI, WGA.mov | 9.23 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_Isoform 2_merge_AF488, DAPI, WGA.mov | 6.21 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_Isoform 3_individual_AF488, DAPI, WGA.mov | 6.51 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_Isoform 3_merge_AF488, DAPI, WGA.mov | 4.65 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_NPR-A_individual_AF488, DAPI, WGA.mov | 3.91 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_NPR-A_merge_AF488, DAPI, WGA.mov | 2.68 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_untransfected_individual_AF488, DAPI, WGA.mov | 1.05 MB | Video Quicktime | OPEN | None | Preview online | |
Immunofluorescence_untransfected_merge_AF488, DAPI, WGA.mov | 818.97 kB | Video Quicktime | OPEN | None | Preview online |
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