Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/235782
Title: LEVERAGING PAN-GENOMES TO UNDERSTAND HIGHLY POLYMORPHIC NLRS
Authors: RACHELLE LEE RUI QI
ORCID iD:   orcid.org/0000-0003-3267-2699
Keywords: pangenome,bioinformatics,plants,NLR,CRISPR,genomics
Issue Date: 15-Aug-2022
Citation: RACHELLE LEE RUI QI (2022-08-15). LEVERAGING PAN-GENOMES TO UNDERSTAND HIGHLY POLYMORPHIC NLRS. ScholarBank@NUS Repository.
Abstract: Pan-genomics is a relatively new approach to genomics that focuses on the repertoire of genomic features in a taxon instead of a reference genome, and therefore is particularly useful for gene families exhibiting significant population-wide variation, such as plant nucleotide-binding leucine-rich repeat (NLR) receptors. NLRs are key players of innate immunity and are abundant in land plant genomes. Unfortunately, NLRs frequently participate in hybrid necrosis, a phenomenon where hybrid offsprings are unusually weak often due to detrimental interactions between genetic elements from different parents. Using recently published, publicly available pan-genomic resources for the model plant Arabidopsis thaliana, I conducted bioinformatic analyses with the goal of understanding the risk factors associated with hybrid necrosis, as well as identifying novel, potential incompatibilities among NLRs. Additionally, I described a CRISPR gRNA design tool that generates small, reusable gRNA panels for pan-genomic experiments on highly polymorphic genes, for which reference-based gRNA may be inadequate.
URI: https://scholarbank.nus.edu.sg/handle/10635/235782
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