Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/235017
Title: SINGLE-CELL BIOCHEMICAL PROFILING OF LEUKOCYTES FROM WHOLE BLOOD
Authors: LU RI
ORCID iD:   orcid.org/0000-0001-8850-6644
Keywords: immune response, leukocyte secretomics, integrated microfludics, acute myocardial infarction, CAR-T cell therapy
Issue Date: 3-Aug-2022
Citation: LU RI (2022-08-03). SINGLE-CELL BIOCHEMICAL PROFILING OF LEUKOCYTES FROM WHOLE BLOOD. ScholarBank@NUS Repository.
Abstract: Secreted enzymes and cytokines reflect functionality of leukocytes. Single-cell assay is needed to identify precise cell subsets with high functional activity. A major challenge for measuring single-cell secretion from blood is the time-consuming and unphysiological sample preparation processes. To address this challenge, this interdisciplinary research developed a novel system for 40 min 1-stop assay from whole blood to test single-leukocyte for up to 5 targets of surface antigen, secreted enzyme and cytokine. First, an integrated microfluidic device was developed automates 6 steps of leukocyte extraction, washing, reagent filling, single-cell encapsulation, incubation and profiling. Next, a customized cytometer system was built to enable novel assay for sensitive detection of secreted enzyme and cytokine. Finally, the system was validated by 2 applications: (1) enzyme secretion and surface antigen quantification evaluating inflammation in acute myocardial infarction (AMI) (2) cytokine secretion test for engineered chimeric antigen receptor (CAR) T cell.
URI: https://scholarbank.nus.edu.sg/handle/10635/235017
Appears in Collections:Ph.D Theses (Restricted)

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