Please use this identifier to cite or link to this item: https://doi.org/10.3390/microorganisms9102128
Title: Evaluation of tetracycline resistance and determination of the tentative microbiological cutoff values in lactic acid bacterial species
Authors: Ma, Qingqing
Pei, Zhangming
Fang, Zhifeng
Wang, Hongchao
Zhu, Jinlin
Lee, Yuan-kun 
Zhang, Hao
Zhao, Jianxin
Lu, Wenwei
Chen, Wei
Keywords: Lactic acid bacteria
Microbiological cutoff value
Minimum inhibitory concentration
Tetracycline resistance
Tetracycline resistance gene
Issue Date: 11-Oct-2021
Publisher: MDPI
Citation: Ma, Qingqing, Pei, Zhangming, Fang, Zhifeng, Wang, Hongchao, Zhu, Jinlin, Lee, Yuan-kun, Zhang, Hao, Zhao, Jianxin, Lu, Wenwei, Chen, Wei (2021-10-11). Evaluation of tetracycline resistance and determination of the tentative microbiological cutoff values in lactic acid bacterial species. Microorganisms 9 (10) : 2128. ScholarBank@NUS Repository. https://doi.org/10.3390/microorganisms9102128
Rights: Attribution 4.0 International
Abstract: Lactic acid bacteria (LAB) are widely used as probiotics in the food industry owing to their beneficial effects on human health. However, numerous antibiotic resistance genes have been found in LAB strains, especially tetracycline resistance genes. Notably, the potential transferability of these genes poses safety risks. To comprehensively evaluate tetracycline resistance in LAB, we determined the tetracycline susceptibility patterns of 478 LAB strains belonging to four genera and eight species. By comparing phenotypes with genotypes based on genome-wide annotations, five tetracycline resistance genes, tet(M), tet(W/N/W), tet(L), tet(S), and tet(45), were detected in LAB. Multiple LAB strains without tetracycline resistance genes were found to be resistant to tetracycline at the currently recommended cutoff values. Thus, based on the minimum inhibitory concentrations of tetracycline for these LAB strains, the species-specific microbiological cutoff values for Lactobacillus (para)gasseri, Lactobacillus johnsonii, and Lactobacillus crispatus to tetracycline were first developed using the Turnidge, Kronvall, and eyeball methods. The cutoff values for Lactiplantibacillus plantarum were re-established and could be used to better distinguish susceptible strains from strains with acquired resistance. Finally, we verified that these five genes play a role in tetracycline resistance and found that tet(M) and tet(W/N/W) are the most widely distributed tetracycline resistance genes in LAB. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Microorganisms
URI: https://scholarbank.nus.edu.sg/handle/10635/233638
ISSN: 2076-2607
DOI: 10.3390/microorganisms9102128
Rights: Attribution 4.0 International
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