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Title: NAD modulates dna methylation and cell differentiation
Authors: Ummarino, Simone
Hausman, Clinton
Gaggi, Giulia
Rinaldi, Lucrezia
Bassal, Mahmoud A. 
Zhang, Yanzhou
Seelam, Andy Joe
Kobayashi, Ikei S.
Borchiellini, Marta
Ebralidze, Alexander K.
Ghinassi, Barbara
Trinh, Bon Q.
Kobayashi, Susumu S.
Di Ruscio, Annalisa
Keywords: DNA methylation
Gene regulation
Issue Date: 2-Nov-2021
Publisher: MDPI
Citation: Ummarino, Simone, Hausman, Clinton, Gaggi, Giulia, Rinaldi, Lucrezia, Bassal, Mahmoud A., Zhang, Yanzhou, Seelam, Andy Joe, Kobayashi, Ikei S., Borchiellini, Marta, Ebralidze, Alexander K., Ghinassi, Barbara, Trinh, Bon Q., Kobayashi, Susumu S., Di Ruscio, Annalisa (2021-11-02). NAD modulates dna methylation and cell differentiation. Cells 10 (11) : 2986. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Nutritional intake impacts the human epigenome by directing epigenetic pathways in normal cell development via as yet unknown molecular mechanisms. Consequently, imbalance in the nutritional intake is able to dysregulate the epigenetic profile and drive cells towards malignant transformation. Here we present a novel epigenetic effect of the essential nutrient, NAD. We demonstrate that impairment of DNMT1 enzymatic activity by NAD?promoted ADP?ribosylation leads to demethylation and transcriptional activation of the CEBPA gene, suggesting the existence of an unknown NAD?controlled region within the locus. In addition to the molecular events, NAD-treated cells exhibit significant morphological and phenotypical changes that correspond to mye-loid differentiation. Collectively, these results delineate a novel role for NAD in cell differ-entiation, and indicate novel nutri?epigenetic strategies to regulate and control gene expression in human cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Cells
ISSN: 2073-4409
DOI: 10.3390/cells10112986
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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