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Title: Extracellular domains of E-cadherin determine key mechanical phenotypes of an epithelium through cell- And non-cellautonomous outside-in signaling
Authors: Aladin, Darwesh Mohideen Kaderbatcha 
Chu, Yeh Shiu
Shen, Shuo
Robinson, Robert Charles
Dufour, Sylvie
Viasnoff, Virgile 
Borghi, Nicolas
Thiery, Jean Paul 
Issue Date: 22-Dec-2021
Publisher: Public Library of Science
Citation: Aladin, Darwesh Mohideen Kaderbatcha, Chu, Yeh Shiu, Shen, Shuo, Robinson, Robert Charles, Dufour, Sylvie, Viasnoff, Virgile, Borghi, Nicolas, Thiery, Jean Paul (2021-12-22). Extracellular domains of E-cadherin determine key mechanical phenotypes of an epithelium through cell- And non-cellautonomous outside-in signaling. PLoS ONE 16 (12-Dec) : e0260593. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Cadherins control intercellular adhesion in most metazoans. In vertebrates, intercellular adhesion differs considerably between cadherins of type-I and type-II, predominantly due to their different extracellular regions. Yet, intercellular adhesion critically depends on actomyosin contractility, in which the role of the cadherin extracellular region is unclear. Here, we dissect the roles of the Extracellular Cadherin (EC) Ig-like domains by expressing chimeric E-cadherin with E-cadherin and cadherin-7 Ig-like domains in cells naturally devoid of cadherins. Using cell-cell separation, cortical tension measurement, tissue stretching and migration assays, we show that distinct EC repeats in the extracellular region of cadherins differentially modulate epithelial sheet integrity, cell-cell separation forces, and cell cortical tension with the Cdc42 pathway, which further differentially regulate epithelial tensile strength, ductility, and ultimately collective migration. Interestingly, dissipative processes rather than static adhesion energy mostly dominate cell-cell separation forces. We provide a framework for the emergence of epithelial phenotypes from cell mechanical properties dependent on EC outside-in signaling. © 2021 Aladin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0260593
Rights: Attribution 4.0 International
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