Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biopha.2021.111328
Title: Designing and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-?-specific nanobody
Authors: Nie, Jifan
Ma, Xingyuan
Hu, Fabiao
Miao, Hui
Feng, Xin
Zhang, Peiwen
Han, Myong Hun
You, Fang 
Yang, Yi
Zhang, Wenlian
Zheng, Wenyun
Keywords: Designing and constructing
Humanized TNF-?-specific Nb
Phage display library
Screening and identifyin
Single domain antibodies
Issue Date: 1-May-2021
Publisher: Elsevier Masson s.r.l.
Citation: Nie, Jifan, Ma, Xingyuan, Hu, Fabiao, Miao, Hui, Feng, Xin, Zhang, Peiwen, Han, Myong Hun, You, Fang, Yang, Yi, Zhang, Wenlian, Zheng, Wenyun (2021-05-01). Designing and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-?-specific nanobody. Biomedicine and Pharmacotherapy 137 : 111328. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biopha.2021.111328
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Tumor necrosis factor (TNF-?) is an important clinically tested cytokine that could induce autoimmune diseases and inflammation. Therefore, the anti-TNF-? therapy strategy was developed and used therapeutically in various diseases, especially in the cytokine storm associated chimeric antigen receptor (CAR) T-cell therapy and antiviral therapy. Compare with other anti-TNF-? inhibitors, anti-TNF-? Nb (nanobody) has many unique advantages. Herein, we reported a novel humanized scaffold for library construction, which could be soluble and expressed in Escherichia coli (E.coli), and the efficiency capacity could reach as high as 2.01 × 109. Meanwhile, an anti-TNF-? Nb was selected for further study after 4 rounds of screening, NT-3, as the optimal Nb could effectively inhibit TNF-mediated cytotoxicity. The IC50 of NT-3 was determined as 0.804 ?M, and its apoptosis inhibition rate was 62.47 % in L929 cells. Furthermore, the molecular docking results showed that complementarity-determining regions (CDRs) of NT-3 could connect to TNF for blocking function through strong hydrogen bonds and salt bridges. In general, our study not only provided a good Nb screening platform in vitro without animal immunization, but also generated a series of novel humanized anti-TNF-? Nb candidates with potential applications. © 2021
Source Title: Biomedicine and Pharmacotherapy
URI: https://scholarbank.nus.edu.sg/handle/10635/233206
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2021.111328
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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