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Title: Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion
Authors: Le, Anh Phuong 
Rupprecht, Jean-Francois
Mège, René-Marc
Toyama, Yusuke 
Lim, Chwee Teck 
Ladoux, Benoit
Issue Date: 15-Jan-2021
Publisher: Nature Research
Citation: Le, Anh Phuong, Rupprecht, Jean-Francois, Mège, René-Marc, Toyama, Yusuke, Lim, Chwee Teck, Ladoux, Benoit (2021-01-15). Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion. Nature Communications 12 (1) : 397. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Apoptotic extrusion is crucial in maintaining epithelial homeostasis. Current literature supports that epithelia respond to extrusion by forming a supracellular actomyosin purse-string in the neighbors. However, whether other actin structures could contribute to extrusion and how forces generated by these structures can be integrated are unknown. Here, we found that during extrusion, a heterogeneous actin network composed of lamellipodia protrusions and discontinuous actomyosin cables, was reorganized in the neighboring cells. The early presence of basal lamellipodia protrusion participated in both basal sealing of the extrusion site and orienting the actomyosin purse-string. The co-existence of these two mechanisms is determined by the interplay between the cell-cell and cell-substrate adhesions. A theoretical model integrates these cellular mechanosensitive components to explain why a dual-mode mechanism, which combines lamellipodia protrusion and purse-string contractility, leads to more efficient extrusion than a single-mode mechanism. In this work, we provide mechanistic insight into extrusion, an essential epithelial homeostasis process. © 2021, The Author(s).
Source Title: Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-020-20563-9
Rights: Attribution 4.0 International
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