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Title: Comprehensive mapping of SARS-CoV-2 interactions in vivo reveals functional virus-host interactions
Authors: Yang, Siwy Ling
Defalco, Louis
Anderson, Danielle E. 
Zhang, Yu
Aw, Jong Ghut Ashley
Lim, Su Ying
Lim, Xin Ni
Tan, Kiat Yee
Zhang, Tong
Chawla, Tanu 
Su, Yan
Lezhava, Alexander
Merits, Andres
Wang, Lin-Fa 
Huber, Roland G.
Wan, Yue 
Issue Date: 25-Aug-2021
Publisher: Nature Research
Citation: Yang, Siwy Ling, Defalco, Louis, Anderson, Danielle E., Zhang, Yu, Aw, Jong Ghut Ashley, Lim, Su Ying, Lim, Xin Ni, Tan, Kiat Yee, Zhang, Tong, Chawla, Tanu, Su, Yan, Lezhava, Alexander, Merits, Andres, Wang, Lin-Fa, Huber, Roland G., Wan, Yue (2021-08-25). Comprehensive mapping of SARS-CoV-2 interactions in vivo reveals functional virus-host interactions. Nature Communications 12 (1) : 5113. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: SARS-CoV-2 is a major threat to global health. Here, we investigate the RNA structure and RNA-RNA interactions of wildtype (WT) and a mutant (?382) SARS-CoV-2 in cells using Illumina and Nanopore platforms. We identify twelve potentially functional structural elements within the SARS-CoV-2 genome, observe that subgenomic RNAs can form different structures, and that WT and ?382 virus genomes fold differently. Proximity ligation sequencing identify hundreds of RNA-RNA interactions within the virus genome and between the virus and host RNAs. SARS-CoV-2 genome binds strongly to mitochondrial and small nucleolar RNAs and is extensively 2’-O-methylated. 2’-O-methylation sites are enriched in viral untranslated regions, associated with increased virus pair-wise interactions, and are decreased in host mRNAs upon virus infection, suggesting that the virus sequesters methylation machinery from host RNAs towards its genome. These studies deepen our understanding of the molecular and cellular basis of SARS-CoV-2 pathogenicity and provide a platform for targeted therapy. © 2021, The Author(s).
Source Title: Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-021-25357-1
Rights: Attribution 4.0 International
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