Please use this identifier to cite or link to this item: https://doi.org/10.3390/cimb43030131
Title: Evaluation of anti-inflammatory effects of celery leaf and stem extracts in lps-induced raw 264.7 cells using nitric oxide assay and lc-ms based metabolomics
Authors: Lau, Hazel
Ni, Nengyi
Dayal, Hiranya 
Lim, Si-Ying
Ren, Yi
Li, Sam Fong-Yau 
Keywords: Celery
Inflammation
LC-MS
Metabolomics
Plant
RAW 264.7 cells
Issue Date: 5-Nov-2021
Publisher: MDPI
Citation: Lau, Hazel, Ni, Nengyi, Dayal, Hiranya, Lim, Si-Ying, Ren, Yi, Li, Sam Fong-Yau (2021-11-05). Evaluation of anti-inflammatory effects of celery leaf and stem extracts in lps-induced raw 264.7 cells using nitric oxide assay and lc-ms based metabolomics. Current Issues in Molecular Biology 43 (3) : 1876-1888. ScholarBank@NUS Repository. https://doi.org/10.3390/cimb43030131
Rights: Attribution 4.0 International
Abstract: The present work demonstrated and compared the anti-inflammatory effects of celery leaf (CLE) and stem (CSE) extracts. LC-MS-based metabolomics were an effective approach to achieve the biomarker identification and pathway elucidation associated with the reduction in inflammatory responses. The celery extracts suppressed LPS-induced NO production in RAW 264.7 cells, and CLE was five times more effective than CSE. Distinct differences were revealed between the control and celery-treated samples among the 24 characteristic metabolites that were identified. In celery-treated LPS cells, reversals of intracellular (citrulline, proline, creatine) and extracellular (citrulline, lysine) metabolites revealed that the therapeutic outcomes were closely linked to arginine metabolism. Reversals of metabolites when treated with CLE (aspartate, proline) indicated targeted effects on the TCA and urea cycles, while, in the case of CSE (histidine, glucose), the glycolysis and the pentose phosphate pathways were implicated. Subsequently, apigenin and bergapten in CLE were identified as potential biomarkers mediating the anti-inflammatory response. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Current Issues in Molecular Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/232697
ISSN: 1467-3037
DOI: 10.3390/cimb43030131
Rights: Attribution 4.0 International
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