Please use this identifier to cite or link to this item: https://doi.org/10.3390/d13080350
Title: Digital droplet pcr-based environmental dna tool for monitoring cryptocaryon irritans in a marine fish farm from Hong Kong
Authors: Tsang, Hin Hung
Domingos, Jose A.
Westaway, Jacob A. F.
Kam, Maximilian H. Y.
Huerlimann, Roger
Bastos Gomes, Giana
Keywords: Aquaculture
Bacterial microbiome
Biomonitoring
Ciliate parasites
EDNA
Marine fish parasite
Issue Date: 29-Jul-2021
Publisher: MDPI
Citation: Tsang, Hin Hung, Domingos, Jose A., Westaway, Jacob A. F., Kam, Maximilian H. Y., Huerlimann, Roger, Bastos Gomes, Giana (2021-07-29). Digital droplet pcr-based environmental dna tool for monitoring cryptocaryon irritans in a marine fish farm from Hong Kong. Diversity 13 (8) : 350. ScholarBank@NUS Repository. https://doi.org/10.3390/d13080350
Rights: Attribution 4.0 International
Abstract: The adoption of new investigative strategies based on environmental DNA (eDNA) can be used to monitor parasites, associated bacterial microbiomes, and physical-chemical parameters in fish farms. In this study, we used the economically important and globally distributed fish ciliate parasite Cryptocaryon irritans as a model to understand the parasite abundance and potential drivers of its presence in marine fish farms. Environmental (rainfall) and physical-chemical (temperature, oxygen, salinity, pH) data collected from a marine fish farm in Hong Kong were analyzed together with the eDNA approach targeting C. irritans abundance based on digital droplet PCR and 16S metagenomics to determine associations and triggers between parasites and specific bacterial groups. Rainfall and temperature demonstrated positive associations with high abundance of C. irritans (eDNA) at the studied marine fish cage farm. However, rainfall was the only parameter tested that demonstrated a significant association with parasite eDNA, indicating that the raining season is a risky period for fish farmers in Hong Kong. Coraliomargarita was the bacterial genus with the most significant relationship with low abundance of C. irritans in water. Understanding the environmental triggers of ciliate parasites propagation and associated bacterial microbiome could elucidate new insights into environmental control, microbial management, and promote the reduction of chemical use in marine fish farms. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Diversity
URI: https://scholarbank.nus.edu.sg/handle/10635/232651
ISSN: 1424-2818
DOI: 10.3390/d13080350
Rights: Attribution 4.0 International
Appears in Collections:Department Publications

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