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Title: Plasma metabolome and lipidome associations with type 2 diabetes and diabetic nephropathy
Authors: Tan, Yan Ming 
Gao, Yan
Teo, Guoshou 
Koh, Hiromi W. L. 
Tai, E. Shyong 
Khoo, Chin Meng 
Choi, Kwok Pui 
Zhou, Lei 
Choi, Hyungwon 
Keywords: Data independent acquisition
Diabetic nephropathy
Oxidative stress
Uremic toxins
Issue Date: 8-Apr-2021
Publisher: MDPI AG
Citation: Tan, Yan Ming, Gao, Yan, Teo, Guoshou, Koh, Hiromi W. L., Tai, E. Shyong, Khoo, Chin Meng, Choi, Kwok Pui, Zhou, Lei, Choi, Hyungwon (2021-04-08). Plasma metabolome and lipidome associations with type 2 diabetes and diabetic nephropathy. Metabolites 11 (4) : 228. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: We conducted untargeted metabolomics analysis of plasma samples from a cross-sectional case–control study with 30 healthy controls, 30 patients with diabetes mellitus and normal renal function (DM-N), and 30 early diabetic nephropathy (DKD) patients using liquid chromatography-mass spectrometry (LC-MS). We employed two different modes of MS acquisition on a high-resolution MS instrument for identification and semi-quantification, and analyzed data using an advanced multivariate method for prioritizing differentially abundant metabolites. We obtained semi-quantification data for 1088 unique compounds (~55% lipids), excluding compounds that may be either exogenous compounds or treated as medication. Supervised classification analysis over a confounding-free partial correlation network shows that prostaglandins, phospholipids, nucleotides, sugars, and glycans are elevated in the DM-N and DKD patients, whereas glutamine, phenylacetylglutamine, 3-indoxyl sulfate, acetylphenylalanine, xanthine, dimethyluric acid, and asymmetric dimethylarginine are increased in DKD compared to DM-N. The data recapitulate the well-established plasma metabolome changes associated with DM-N and suggest uremic solutes and oxidative stress markers as the compounds indicating early renal function decline in DM patients. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Metabolites
ISSN: 2218-1989
DOI: 10.3390/metabo11040228
Rights: Attribution 4.0 International
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