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dc.titleHybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study
dc.contributor.authorZhao, Yu
dc.contributor.authorLiu, Shiqi
dc.contributor.authorLu, Zhihui
dc.contributor.authorZhao, Baixiang
dc.contributor.authorWang, Shuhui
dc.contributor.authorZhang, Cuiying
dc.contributor.authorXiao, Dongguang
dc.contributor.authorFoo, Jee Loon
dc.contributor.authorYu, Aiqun
dc.identifier.citationZhao, Yu, Liu, Shiqi, Lu, Zhihui, Zhao, Baixiang, Wang, Shuhui, Zhang, Cuiying, Xiao, Dongguang, Foo, Jee Loon, Yu, Aiqun (2021-07-02). Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study. Biotechnology for Biofuels 14 (1) : 149. ScholarBank@NUS Repository.
dc.description.abstractBackground: In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level of gene expression. Therefore, rational fine-tuning of promoters to regulate the expression levels of target genes for optimizing biosynthetic pathways in metabolic engineering has recently become an active area of research. Results: In this study, we systematically detected and characterized the common promoter elements in the unconventional yeast Yarrowia lipolytica, and constructed an artificial hybrid promoter library that covers a wide range of promoter strength. The results indicate that the hybrid promoter strength can be fine-tuned by promoter elements, namely, upstream activation sequences (UAS), TATA box and core promoter. Notably, the UASs of Saccharomyces cerevisiae promoters were reported for the first time to be functionally transferred to Y. lipolytica. Subsequently, using the production of a versatile platform chemical isoamyl alcohol as a test study, the hybrid promoter library was applied to optimize the biosynthesis pathway expression in Y. lipolytica. By expressing the key pathway gene, ScARO10, with the promoter library, 1.1–30.3 folds increase in the isoamyl alcohol titer over that of the control strain Y. lipolytica Po1g KU70? was achieved. Interestingly, the highest titer increase was attained with a weak promoter PUAS1B4-EXPm to express ScARO10. These results suggest that our hybrid promoter library can be a powerful toolkit for identifying optimum promoters for expressing metabolic pathways in Y. lipolytica. Conclusion: We envision that this promoter engineering strategy and the rationally engineered promoters constructed in this study could also be extended to other non-model fungi for strain improvement. © 2021, The Author(s).
dc.publisherBioMed Central Ltd
dc.rightsAttribution 4.0 International
dc.sourceScopus OA2021
dc.subjectHybrid promoter
dc.subjectIsoamyl alcohol
dc.subjectMetabolic engineering
dc.subjectSynthetic promoter
dc.subjectY. lipolytica
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.sourcetitleBiotechnology for Biofuels
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