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Title: The retromer CSC subcomplex is recruited by MoYpt7 and sequentially sorted by MoVps17 for effective conidiation and pathogenicity of the rice blast fungus
Authors: Wu, Congxian
Lin, Yahong
Zheng, Huawei
Abubakar, Yakubu Saddeeq
Peng, Minghui
Li, Jingjing
Yu, Zhi
Wang, Zonghua
Naqvi, Naweed I. 
Li, Guangpu
Zhou, Jie
Zheng, Wenhui
Keywords: Magnaporthe oryzae
Issue Date: 21-Dec-2020
Publisher: Blackwell Publishing Ltd
Citation: Wu, Congxian, Lin, Yahong, Zheng, Huawei, Abubakar, Yakubu Saddeeq, Peng, Minghui, Li, Jingjing, Yu, Zhi, Wang, Zonghua, Naqvi, Naweed I., Li, Guangpu, Zhou, Jie, Zheng, Wenhui (2020-12-21). The retromer CSC subcomplex is recruited by MoYpt7 and sequentially sorted by MoVps17 for effective conidiation and pathogenicity of the rice blast fungus. Molecular Plant Pathology 22 (2) : 284-298. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: In eukaryotic cells, Rab GTPases and the retromer complex are important regulators of intracellular protein transport. However, the mechanistic relationship between Rab GTPases and the retromer complex in relation to filamentous fungal development and pathogenesis is unknown. In this study, we used Magnaporthe oryzae, an important pathogen of rice and other cereals, as a model filamentous fungus to dissect this knowledge gap. Our data demonstrate that the core retromer subunit MoVps35 interacts with the Rab GTPase MoYpt7 and they colocalize to the endosome. Without MoYpt7, MoVps35 is mislocalized in the cytoplasm, indicating that MoYpt7 plays an important role in the recruitment of MoVps35. We further demonstrate that the expression of an inactive MoYpt7-DN (GDP-bound form) mutant in M. oryzae mimicks the phenotype defects of retromer cargo-sorting complex (CSC) null mutants and blocks the proper localization of MoVps35. In addition, our data establish that MoVps17, a member of the sorting nexin family, is situated at the endosome independent of retromer CSC but regulates the sorting function of MoVps35 after its recruitment to the endosomal membrane by MoYpt7. Taken together, these results provide insight into the precise mechanism of retromer CSC recruitment to the endosome by MoYpt7 and subsequent sorting by MoVps17 for efficient conidiation and pathogenicity of M. oryzae. © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd
Source Title: Molecular Plant Pathology
ISSN: 1464-6722
DOI: 10.1111/mpp.13029
Rights: Attribution 4.0 International
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