Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms22063278
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dc.titleUtilizing an animal model to identify brain neurodegeneration-related biomarkers in aging
dc.contributor.authorYang, Ming-Hui
dc.contributor.authorChen, Yi-Ming Arthur
dc.contributor.authorTu, Shan-Chen
dc.contributor.authorChi, Pei-Ling
dc.contributor.authorChuang, Kuo-Pin
dc.contributor.authorChang, Chin-Chuan
dc.contributor.authorLee, Chiang-Hsuan
dc.contributor.authorChen, Yi-Ling
dc.contributor.authorLee, Che-Hsin
dc.contributor.authorYuan, Cheng-Hui
dc.contributor.authorTyan, Yu-Chang
dc.date.accessioned2022-10-11T08:01:26Z
dc.date.available2022-10-11T08:01:26Z
dc.date.issued2021-03-23
dc.identifier.citationYang, Ming-Hui, Chen, Yi-Ming Arthur, Tu, Shan-Chen, Chi, Pei-Ling, Chuang, Kuo-Pin, Chang, Chin-Chuan, Lee, Chiang-Hsuan, Chen, Yi-Ling, Lee, Che-Hsin, Yuan, Cheng-Hui, Tyan, Yu-Chang (2021-03-23). Utilizing an animal model to identify brain neurodegeneration-related biomarkers in aging. International Journal of Molecular Sciences 22 (6) : 3278. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms22063278
dc.identifier.issn1661-6596
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/232121
dc.description.abstractGlycine N-methyltransferase (GNMT) regulates S-adenosylmethionine (SAMe), a methyl donor in methylation. Over-expressed SAMe may cause neurogenic capacity reduction and memory impairment. GNMT knockout mice (GNMT-KO) was applied as an experimental model to evaluate its effect on neurons. In this study, proteins from brain tissues were studied using proteo-mic approaches, Haemotoxylin and Eosin staining, immunohistochemistry, Western blotting, and ingenuity pathway analysis. The expression of Receptor-interacting protein 1(RIPK1) and Caspase 3 were up-regulated and activity-dependent neuroprotective protein (ADNP) was down-regulated in GNMT-KO mice regardless of the age. Besides, proteins related to neuropathology, such as ex-citatory amino acid transporter 2, calcium/calmodulin-dependent protein kinase type II subunit al-pha, and Cu-Zn superoxide dismutase were found only in the group of aged wild-type mice; 4-aminobutyrate amino transferase, limbic system-associated membrane protein, sodium-and chlo-ride-dependent GABA transporter 3 and ProSAAS were found only in the group of young GNMT-KO mice and are related to function of neurons; serum albumin and Rho GDP dissociation inhibitor 1 were found only in the group of aged GNMT-KO mice and are connected to neurodegenerative disorders. With proteomic analyses, a pathway involving Gonadotropin-releasing hormone (GnRH) signal was found to be associated with aging. The GnRH pathway could provide additional information on the mechanism of aging and non-aging related neurodegeneration, and these protein markers may be served in developing future therapeutic treatments to ameliorate aging and prevent diseases. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2021
dc.subjectAging
dc.subjectBiomarker
dc.subjectGNMT
dc.subjectNeurons
dc.typeArticle
dc.contributor.departmentDEPT OF CHEMISTRY
dc.description.doi10.3390/ijms22063278
dc.description.sourcetitleInternational Journal of Molecular Sciences
dc.description.volume22
dc.description.issue6
dc.description.page3278
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