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Title: Utilizing an animal model to identify brain neurodegeneration-related biomarkers in aging
Authors: Yang, Ming-Hui
Chen, Yi-Ming Arthur
Tu, Shan-Chen
Chi, Pei-Ling
Chuang, Kuo-Pin
Chang, Chin-Chuan
Lee, Chiang-Hsuan
Chen, Yi-Ling
Lee, Che-Hsin
Yuan, Cheng-Hui 
Tyan, Yu-Chang
Keywords: Aging
Issue Date: 23-Mar-2021
Publisher: MDPI AG
Citation: Yang, Ming-Hui, Chen, Yi-Ming Arthur, Tu, Shan-Chen, Chi, Pei-Ling, Chuang, Kuo-Pin, Chang, Chin-Chuan, Lee, Chiang-Hsuan, Chen, Yi-Ling, Lee, Che-Hsin, Yuan, Cheng-Hui, Tyan, Yu-Chang (2021-03-23). Utilizing an animal model to identify brain neurodegeneration-related biomarkers in aging. International Journal of Molecular Sciences 22 (6) : 3278. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Glycine N-methyltransferase (GNMT) regulates S-adenosylmethionine (SAMe), a methyl donor in methylation. Over-expressed SAMe may cause neurogenic capacity reduction and memory impairment. GNMT knockout mice (GNMT-KO) was applied as an experimental model to evaluate its effect on neurons. In this study, proteins from brain tissues were studied using proteo-mic approaches, Haemotoxylin and Eosin staining, immunohistochemistry, Western blotting, and ingenuity pathway analysis. The expression of Receptor-interacting protein 1(RIPK1) and Caspase 3 were up-regulated and activity-dependent neuroprotective protein (ADNP) was down-regulated in GNMT-KO mice regardless of the age. Besides, proteins related to neuropathology, such as ex-citatory amino acid transporter 2, calcium/calmodulin-dependent protein kinase type II subunit al-pha, and Cu-Zn superoxide dismutase were found only in the group of aged wild-type mice; 4-aminobutyrate amino transferase, limbic system-associated membrane protein, sodium-and chlo-ride-dependent GABA transporter 3 and ProSAAS were found only in the group of young GNMT-KO mice and are related to function of neurons; serum albumin and Rho GDP dissociation inhibitor 1 were found only in the group of aged GNMT-KO mice and are connected to neurodegenerative disorders. With proteomic analyses, a pathway involving Gonadotropin-releasing hormone (GnRH) signal was found to be associated with aging. The GnRH pathway could provide additional information on the mechanism of aging and non-aging related neurodegeneration, and these protein markers may be served in developing future therapeutic treatments to ameliorate aging and prevent diseases. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
ISSN: 1661-6596
DOI: 10.3390/ijms22063278
Rights: Attribution 4.0 International
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