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Title: A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
Authors: Goh, Yun Shan
Ng, Lisa F.P. 
Renia, Laurent
Keywords: Antibody
Cell Biology
Flow Cytometry/Mass Cytometry
Health Sciences
Molecular Biology
Issue Date: 1-Sep-2021
Publisher: Cell Press
Citation: Goh, Yun Shan, Ng, Lisa F.P., Renia, Laurent (2021-09-01). A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients. STAR Protocols 2 (3) : 100671. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021). © 2021 Agency for Science, Technology and Research
Source Title: STAR Protocols
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100671
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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