Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2021.731410
Title: Potential Probiotic Pediococcus pentosaceus M41 Modulates Its Proteome Differentially for Tolerances Against Heat, Cold, Acid, and Bile Stresses
Authors: Baig, Mohd Affan
Turner, Mark S.
Liu, Shao-Quan 
Al-Nabulsi, Anas A.
Shah, Nagendra P.
Ayyash, Mutamed M.
Keywords: acid stress
bacterial proteins
bile stress
environmental stress
nano-LC-MS/MS
proteomics
Issue Date: 13-Oct-2021
Publisher: Frontiers Media S.A.
Citation: Baig, Mohd Affan, Turner, Mark S., Liu, Shao-Quan, Al-Nabulsi, Anas A., Shah, Nagendra P., Ayyash, Mutamed M. (2021-10-13). Potential Probiotic Pediococcus pentosaceus M41 Modulates Its Proteome Differentially for Tolerances Against Heat, Cold, Acid, and Bile Stresses. Frontiers in Microbiology 12 : 731410. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2021.731410
Rights: Attribution 4.0 International
Abstract: Probiotics containing functional food confer health benefits in addition to their nutritional properties. In this study, we have evaluated the differential proteomic responses of a potential novel probiotic Pediococcus pentosaceus M41 under heat, cold, acid, and bile stress conditions. We identified stress response proteins that could provide tolerances against these stresses and could be used as probiotic markers for evaluating stress tolerance. Pediococcus pentosaceus M41 was exposed for 2 h to each condition: 50°C (heat stress), 4°C (cold stress), pH 3.0 (acid stress) and 0.05% bile (bile stress). Proteomic analysis was carried out using 2D-IEF SDS PAGE and LC-MS/MS. Out of 60 identified proteins, 14 upregulated and 6 downregulated proteins were common among all the stress conditions. These proteins were involved in different biological functions such as translation-related proteins, carbohydrate metabolism (phosphoenolpyruvate phosphotransferase), histidine biosynthesis (imidazole glycerol phosphate synthase) and cell wall synthesis (tyrosine-protein kinase CapB). Proteins such as polysaccharide deacetylase, lactate oxidase, transcription repressor NrdR, dihydroxyacetone kinase were upregulated under three out of the four stress conditions. The differential expression of these proteins might be responsible for tolerance and protection of P. pentosaceus M41 against different stress conditions. © Copyright © 2021 Baig, Turner, Liu, Al-Nabulsi, Shah and Ayyash.
Source Title: Frontiers in Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/232006
ISSN: 1664-302X
DOI: 10.3389/fmicb.2021.731410
Rights: Attribution 4.0 International
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