Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2021.770109
DC FieldValue
dc.titleRational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol
dc.contributor.authorWang, Xin
dc.contributor.authorZhang, Lin
dc.contributor.authorChen, Hong
dc.contributor.authorWang, Pan
dc.contributor.authorYin, Ying
dc.contributor.authorJin, Jiaqi
dc.contributor.authorXu, Jianwei
dc.contributor.authorWen, Jianping
dc.date.accessioned2022-10-11T07:51:03Z
dc.date.available2022-10-11T07:51:03Z
dc.date.issued2021-11-26
dc.identifier.citationWang, Xin, Zhang, Lin, Chen, Hong, Wang, Pan, Yin, Ying, Jin, Jiaqi, Xu, Jianwei, Wen, Jianping (2021-11-26). Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol. Frontiers in Microbiology 12 : 770109. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2021.770109
dc.identifier.issn1664-302X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/231984
dc.description.abstractIn order to improve the capability of Klebsiella pneumoniae to produce an important chemical raw material, 1,3-propanediol (1,3-PDO), a new type of K. pneumoniae x546 was obtained by glycerol acclimation and subsequently was used to produce 1,3-PDO. Under the control of pH value using Na+ pH neutralizer, the 1,3-PDO yield of K. pneumoniae x546 in a 7.5-L fermenter was 69.35 g/L, which was 1.5-fold higher than the original strain (45.91 g/L). After the addition of betaine, the yield of 1,3-PDO reached up to 74.44 g/L at 24 h, which was 40% shorter than the original fermentation time of 40 h. To study the potential mechanism of the production improvement of 1,3-PDO, the Tandem Mass Tags (TMT) technology was applied to investigate the production of 1,3-PDO in K. pneumoniae. Compared with the control group, 170 up-regulated proteins and 291 down-regulated proteins were identified. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, it was found that some proteins [such as homoserine kinase (ThrB), phosphoribosylglycinamide formyltransferase (PurT), phosphoribosylaminoimidazolesuccinocarboxamide synthase (PurC), etc.] were involved in the fermentation process, whereas some other proteins (such as ProX, ProW, ProV, etc.) played a significant role after the addition of betaine. Moreover, combined with the metabolic network of K. pneumoniae during 1,3-PDO, the proteins in the biosynthesis of 1,3-PDO [such as DhaD, DhaK, lactate dehydrogenase (LDH), BudC, etc.] were analyzed. The process of 1,3-PDO production in K. pneumoniae was explained from the perspective of proteome for the first time, which provided a theoretical basis for genetic engineering modification to improve the yield of 1,3-PDO. Because of the use of Na+ pH neutralizer in the fermentation, the subsequent environmental pollution treatment cost was greatly reduced, showing high potential for industry application in the future. Copyright © 2021 Wang, Zhang, Chen, Wang, Yin, Jin, Xu and Wen.
dc.publisherFrontiers Media S.A.
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2021
dc.subject1
dc.subject3-propanediol production
dc.subjectbetaine
dc.subjectKlebsiella pneumoniae
dc.subjectNa+ pH neutralizer
dc.subjectproteomics
dc.typeArticle
dc.contributor.departmentDEPT OF CHEMISTRY
dc.contributor.departmentCHEMISTRY
dc.description.doi10.3389/fmicb.2021.770109
dc.description.sourcetitleFrontiers in Microbiology
dc.description.volume12
dc.description.page770109
Appears in Collections:Elements
Staff Publications

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_3389_fmicb_2021_770109.pdf7.88 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

1
checked on Jan 30, 2023

Page view(s)

19
checked on Jan 26, 2023

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons