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dc.titleRational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol
dc.contributor.authorWang, Xin
dc.contributor.authorZhang, Lin
dc.contributor.authorChen, Hong
dc.contributor.authorWang, Pan
dc.contributor.authorYin, Ying
dc.contributor.authorJin, Jiaqi
dc.contributor.authorXu, Jianwei
dc.contributor.authorWen, Jianping
dc.identifier.citationWang, Xin, Zhang, Lin, Chen, Hong, Wang, Pan, Yin, Ying, Jin, Jiaqi, Xu, Jianwei, Wen, Jianping (2021-11-26). Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol. Frontiers in Microbiology 12 : 770109. ScholarBank@NUS Repository.
dc.description.abstractIn order to improve the capability of Klebsiella pneumoniae to produce an important chemical raw material, 1,3-propanediol (1,3-PDO), a new type of K. pneumoniae x546 was obtained by glycerol acclimation and subsequently was used to produce 1,3-PDO. Under the control of pH value using Na+ pH neutralizer, the 1,3-PDO yield of K. pneumoniae x546 in a 7.5-L fermenter was 69.35 g/L, which was 1.5-fold higher than the original strain (45.91 g/L). After the addition of betaine, the yield of 1,3-PDO reached up to 74.44 g/L at 24 h, which was 40% shorter than the original fermentation time of 40 h. To study the potential mechanism of the production improvement of 1,3-PDO, the Tandem Mass Tags (TMT) technology was applied to investigate the production of 1,3-PDO in K. pneumoniae. Compared with the control group, 170 up-regulated proteins and 291 down-regulated proteins were identified. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, it was found that some proteins [such as homoserine kinase (ThrB), phosphoribosylglycinamide formyltransferase (PurT), phosphoribosylaminoimidazolesuccinocarboxamide synthase (PurC), etc.] were involved in the fermentation process, whereas some other proteins (such as ProX, ProW, ProV, etc.) played a significant role after the addition of betaine. Moreover, combined with the metabolic network of K. pneumoniae during 1,3-PDO, the proteins in the biosynthesis of 1,3-PDO [such as DhaD, DhaK, lactate dehydrogenase (LDH), BudC, etc.] were analyzed. The process of 1,3-PDO production in K. pneumoniae was explained from the perspective of proteome for the first time, which provided a theoretical basis for genetic engineering modification to improve the yield of 1,3-PDO. Because of the use of Na+ pH neutralizer in the fermentation, the subsequent environmental pollution treatment cost was greatly reduced, showing high potential for industry application in the future. Copyright © 2021 Wang, Zhang, Chen, Wang, Yin, Jin, Xu and Wen.
dc.publisherFrontiers Media S.A.
dc.rightsAttribution 4.0 International
dc.sourceScopus OA2021
dc.subject3-propanediol production
dc.subjectKlebsiella pneumoniae
dc.subjectNa+ pH neutralizer
dc.contributor.departmentDEPT OF CHEMISTRY
dc.description.sourcetitleFrontiers in Microbiology
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