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https://scholarbank.nus.edu.sg/handle/10635/23195
Title: | Effect of radiation on the sensori-neural auditory system and the clinical implications | Authors: | LOW WONG KEIN, CHRISTOPHER | Keywords: | deafness, radiotherapy, chemo-radiotherapy, hair cells, apoptosis, anti-oxidant | Issue Date: | 30-May-2007 | Citation: | LOW WONG KEIN, CHRISTOPHER (2007-05-30). Effect of radiation on the sensori-neural auditory system and the clinical implications. ScholarBank@NUS Repository. | Abstract: | A prospective study on patients with nasopharyngeal carcinoma treated by radiotherapy was conducted. Although the auditory pathways received relatively high radiation doses, the retro-cochlear pathways as measured by brainstem evoked response audiometry, remained functionally intact even up to 4 years after radiation. A single-blinded randomized study on nasopharyngeal carcinoma patients treated with radiotherapy alone and with chemo-radiation therapy was carried out over a 2-year period. It revealed that combined therapy resulted in synergistic ototoxicity, particularly for high frequency sounds in the speech range. In the OC-k3 cochlear cell line, radiation-induced apoptosis was documented by flow cytometry and TUNEL assay. Post-irradiation effects were further studied by microarray analysis, Western blotting and DCFDA detection of ROS. Radiation-induced apoptosis was observed to occur in a dose-dependant and ROS-related fashion with p53 possibly playing a major role. The anti-oxidant L-N-Acetylcysteine was demonstrated to protect the cell line against radiation-induced apoptosis. | URI: | http://scholarbank.nus.edu.sg/handle/10635/23195 |
Appears in Collections: | Ph.D Theses (Open) |
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01ThesisFinal.pdf.pdf | 43.45 kB | Adobe PDF | OPEN | None | View/Download | |
02ThesisFinal.pdf.pdf | 1.35 MB | Adobe PDF | OPEN | None | View/Download |
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