Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12866-021-02226-x
Title: Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice
Authors: Jin Xing
He Yufeng
Zhou Yonghua
Chen Xiaohua
Lee Yuan-Kun 
Zhao Jianxin
Zhang Hao
Chen Wei
Wang Gang
Keywords: Caenorhabditis elegans
Campylobacter jejuni
Immune gene
Lactic acid bacteria
Life-span
Issue Date: 5-Jun-2021
Publisher: BioMed Central Ltd
Citation: Jin Xing, He Yufeng, Zhou Yonghua, Chen Xiaohua, Lee Yuan-Kun, Zhao Jianxin, Zhang Hao, Chen Wei, Wang Gang (2021-06-05). Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice. BMC Microbiology 21 (1) : 169. ScholarBank@NUS Repository. https://doi.org/10.1186/s12866-021-02226-x
Rights: Attribution 4.0 International
Abstract: Background: Campylobacter jejuni is the major micro-bacillary pathogen responsible for human coloenteritis. Lactic acid bacteria (LAB) have been shown to protect against Campylobacter infection. However, LAB with a good ability to inhibit the growth of C. jejuni in vitro are less effective in animals and animal models, and have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for their anti-pathogenic properties. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date. Results: This study established a lifespan model of C. elegans, enabling the preselection of LAB to counter C. jejuni infection. A potential protective mechanism of LAB was identified. Some distinct LAB species offered a high level of protection to C. elegans against C. jejuni. The LAB strains with a high protection rate reduced the load of C. jejuni in C. elegans. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway-related genes was elevated using the LAB isolates with a high protection rate. The reliability of the lifespan model of C. elegans was verified using mice and chickens infected with C. jejuni. Conclusions: The results showed that different LAB had different abilities to protect C. elegans against C. jejuni. C. elegans provides a reliable model for researchers to screen for LAB that are antagonistic to C. jejuni on a large scale. © 2021, The Author(s).
Source Title: BMC Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/231938
ISSN: 1471-2180
DOI: 10.1186/s12866-021-02226-x
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s12866-021-02226-x.pdf1.68 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

1
checked on Nov 21, 2022

Page view(s)

6
checked on Nov 17, 2022

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons