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Title: Molecular analyses of untranslated regions of hibiscus latent Singapore virus
Keywords: HLSV, UTR, Poly(A), translation, PABP, enhancement
Issue Date: 15-Nov-2007
Citation: CAO SHISHU (2007-11-15). Molecular analyses of untranslated regions of hibiscus latent Singapore virus. ScholarBank@NUS Repository.
Abstract: Hibiscus latent Singapore virus (HLSV) is a new tobamovirus recently reported. The genome contains a 5b - and a 3b -untranslated region (UTR). In this study, the functions of 5b UTR and 3b UTR in regulating gene expression were analyzed by in vitro and in vivo assays. In wheat germ extract and kenaf protoplasts, the presence of both 5b UTR and 3b UTR enhanced luciferase activity. Predicted stem loops between 5b UTR and 3b UTR could form nine nucleotide base-pairing which could enhance translation. At the same time, an internal poly(A) tract in the 3b UTR interacted with its 5b cap to form a predicted b closed loopb in enhancing its translation. Therefore, it is suggested that the interaction between the 5b - and 3b -UTRs could promote both poly(A) -dependent and poly(A)-independent translation in HLSV. This study also showed that the length of the internal poly(A) tract and the polyadenylation signal sequence were important for infectivity of HLSV in Nicotiana benthamiana.
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