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|Title:||Fabrication of polyacrylamide micro-pillars and its application in microarray analysis||Authors:||EZREIN SHAH BIN SELAMAT||Keywords:||microarrays, conventional, micro-pillars, waffle mask, soft lithography, diffusivisity||Issue Date:||9-Jul-2007||Citation:||EZREIN SHAH BIN SELAMAT (2007-07-09). Fabrication of polyacrylamide micro-pillars and its application in microarray analysis. ScholarBank@NUS Repository.||Abstract:||To improve diffusive capabilities and target accessibility with a gel-based microarray, a novel a??pads within a pada?? approach was developed. Using a soft lithography technique, an array of a??wafflea??-like micro-pillars embedded on the surface of the glass slide substratum was fabricated. The micro-pillar based microarray showed more than a 3-fold increase in the effective surface area available for probe immobilization. As a result, a 5-fold increase was observed in the hybridization rate and intensity of the a??wafflea?? pad as compared to a conventional gel pad. The functionality of the a??wafflea?? pad was demonstrated by the accurate genotyping of two sets of SNPs (SNP_1236 and SNP_2677) based on the dissociation curve, dissociation temperature, Td and the difference of Td between the perfect match duplex with that of the mismatch (a??Td).||URI:||http://scholarbank.nus.edu.sg/handle/10635/23180|
|Appears in Collections:||Master's Theses (Open)|
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