Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/23178
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dc.titleCloning and characterization of the promoter of the cancer- associated gene, FAT10
dc.contributor.authorZHANG DONGWEI
dc.date.accessioned2011-06-10T18:03:41Z
dc.date.available2011-06-10T18:03:41Z
dc.date.issued2007-05-24
dc.identifier.citationZHANG DONGWEI (2007-05-24). Cloning and characterization of the promoter of the cancer- associated gene, FAT10. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/23178
dc.description.abstractFAT10 was found to play a role in tumorigenesis and over-expression of FAT10 gene may lead to chromosomal stability. To elucidate the mechanism of FAT10 gene regulation, we identified and characterized the FAT10 promoter. We found that the 5a??UTR displayed significant promoter activity. Regions upstream of the 5a??UTR (from +26 to -1997) did not confer any promoter activity. Further experiment elucidated that p53 was found to bind in vivo to the 5a?? half-consensus sequence of p53 binding site located in the FAT10 promoter. Hence, we propose that FAT10 is a downstream target of p53. We proceeded to investigate if the up-regulation of FAT10 expression in the tumors of HCC patients can be accounted for by dysregulation at the FAT10 promoter region. We found that it is DNA methylation, but not mutation in FAT10 promoter region may account for the aberrant expression of FAT10 in HCC patient tissues.
dc.language.isoen
dc.subjectFAT10, promoter, p53, tumerigenesis, methylation, SNP
dc.typeThesis
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.supervisorLEE GUAT LAY, CAROLINE
dc.description.degreeMaster's
dc.description.degreeconferredMASTER OF SCIENCE
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Master's Theses (Open)

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