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Title: Cloning and characterization of the promoter of the cancer- associated gene, FAT10
Keywords: FAT10, promoter, p53, tumerigenesis, methylation, SNP
Issue Date: 24-May-2007
Citation: ZHANG DONGWEI (2007-05-24). Cloning and characterization of the promoter of the cancer- associated gene, FAT10. ScholarBank@NUS Repository.
Abstract: FAT10 was found to play a role in tumorigenesis and over-expression of FAT10 gene may lead to chromosomal stability. To elucidate the mechanism of FAT10 gene regulation, we identified and characterized the FAT10 promoter. We found that the 5a??UTR displayed significant promoter activity. Regions upstream of the 5a??UTR (from +26 to -1997) did not confer any promoter activity. Further experiment elucidated that p53 was found to bind in vivo to the 5a?? half-consensus sequence of p53 binding site located in the FAT10 promoter. Hence, we propose that FAT10 is a downstream target of p53. We proceeded to investigate if the up-regulation of FAT10 expression in the tumors of HCC patients can be accounted for by dysregulation at the FAT10 promoter region. We found that it is DNA methylation, but not mutation in FAT10 promoter region may account for the aberrant expression of FAT10 in HCC patient tissues.
Appears in Collections:Master's Theses (Open)

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