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Title: Post-translational regulation of the human TRIP-Br1 cell cycle protein
Keywords: cell cycle, TRIP-Br, transcriptional regulator, PHD, bromodomain, DP1
Issue Date: 26-Oct-2007
Citation: YANG MAOLIN, CHRISTOPHER (2007-10-26). Post-translational regulation of the human TRIP-Br1 cell cycle protein. ScholarBank@NUS Repository.
Abstract: The TRIP-Br proteins are a novel family of transcriptional regulators proposed to function as a??integratorsa?? at E2F responsive promoters to integrate signals provided by PHD zinc finger- and/or bromodomain-containing transcription factors. Co-immunoprecipitation assays with exogenously co-overexpressed proteins were used to determine interactions between hTRIP-Br1-HA and DP1, E2F1 and/or p53. A putative region for binding to DP1 on hTRIP-Br1 was identified. Conversely, the DP1 DCB1 domain was postulated to be the binding region for hTRIP-Br1,in a review of published data. p53 was also observed to be a component of the E2F1/DP1/hTRIP-Br1-HA quaternary complex.TRIP-Br1 binding partners affected its intracellular protein levels. DP1 interaction with hTRIP-Br1-HA resulted in elevated intracellular protein levels. E2F1 accelerated the turnover of hTRIP-Br1-HA, with or without DP1The results support a regulatory model in which hTRIP-Br1-HA is able to promote DP1 nuclear localization through post-translational mechanisms in a reciprocal manner that influences the activities of both proteins.
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