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Title: Investigating the role of menaquinone metabolism in dormant mycobacteria with antisense RNA
Keywords: mycobacteria, menaquinone, dormancy, antisense, tuberculosis, respiration
Issue Date: 29-May-2007
Citation: THOMAS MICHAEL FIEDLER (2007-05-29). Investigating the role of menaquinone metabolism in dormant mycobacteria with antisense RNA. ScholarBank@NUS Repository.
Abstract: Mycobacteria exclusively use menaquinones in their respiratory chain. The fact that humans rely on ubiquinone and do not have the capability to synthesize menaquinones renders menaquinone metabolism an attractive drug target. We investigate here if menaquinone is essential for bacterial survival during dormancy, by inhibiting the translation of genes coding for menaquinone synthesizing enzymes, through the experimental use of antisense RNA. Differences between transformants and wild-type, concerning the bacteriaa??s ability to survive hypoxia and synthesize menaquinone, were monitored by counting colony forming units (CFU) and measuring levels of menaquinones via mass spectrometry (MS). Based on our observations of cell growth, cells transformed with a plasmid carrying an antisense fragment of the gene entC were compromised in their ability to survive hypoxic conditions. However, an inhibition of menaquinone synthesis and concurrent drops in menaquinone levels could not be confirmed by preliminary MS analysis.
Appears in Collections:Master's Theses (Open)

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