Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/23102
DC FieldValue
dc.titleMultifuctional peptides as biocompatible non-viral vectors for gene delivery
dc.contributor.authorLO SEONG LOONG
dc.date.accessioned2011-06-10T18:02:10Z
dc.date.available2011-06-10T18:02:10Z
dc.date.issued2007-02-28
dc.identifier.citationLO SEONG LOONG (2007-02-28). Multifuctional peptides as biocompatible non-viral vectors for gene delivery. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/23102
dc.description.abstractThe major barrier in gene therapy is the development of gene delivery vectors with cell-type specificity and minimal toxicity. Non-viral vectors are gaining more attention than viral vectors due to the safety consideration in clinical use. To achieve efficient delivery, non-viral vectors are modified to mimic the viral attributes such as interaction with cell surface, membrane fusion, and nuclear localization. In our studies, we constructed biocompatible peptide and polypeptide based vectors for gene delivery. The main features of these vectors are: (1) strong binding and condensation of DNA; (2) favourable positive zeta potential and nanometer-size of DNA/vector complex; (3) efficient endosomal escape of the DNA/vector complex, which is a major limiting factor in gene delivery; (4) insignificant cytotoxicity, which is a main requirement for gene therapy. Hence, these vectors can serve as a platform for developing more efficient vectors by including many different functional domains.
dc.language.isoen
dc.subjectpeptide, Tat, polyethylenimine, histidine, endosomal escape, gene delivery system
dc.typeThesis
dc.contributor.departmentGRADUATE PROGRAMME IN BIOENGINEERING-SOM
dc.contributor.supervisorWANG SHU
dc.contributor.supervisorZHANG YONG
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Lo Seong Loong - PhD Thesis.pdf6.14 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

206
checked on May 21, 2019

Download(s)

199
checked on May 21, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.