Please use this identifier to cite or link to this item: https://doi.org/10.1161/JAHA.121.023038
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dc.titleNitrite Generating and Depleting Capacity of the Oral Microbiome and Cardiometabolic Risk: Results from ORIGINS
dc.contributor.authorGoh, CE
dc.contributor.authorBohn, B
dc.contributor.authorMarotz, C
dc.contributor.authorMolinsky, R
dc.contributor.authorRoy, S
dc.contributor.authorPaster, BJ
dc.contributor.authorChen, CY
dc.contributor.authorRosenbaum, M
dc.contributor.authorYuzefpolskaya, M
dc.contributor.authorColombo, PC
dc.contributor.authorDesvarieux, M
dc.contributor.authorPapapanou, PN
dc.contributor.authorJacobs, DR
dc.contributor.authorKnight, R
dc.contributor.authorDemmer, RT
dc.date.accessioned2022-09-08T07:45:34Z
dc.date.available2022-09-08T07:45:34Z
dc.date.issued2022-05-17
dc.identifier.citationGoh, CE, Bohn, B, Marotz, C, Molinsky, R, Roy, S, Paster, BJ, Chen, CY, Rosenbaum, M, Yuzefpolskaya, M, Colombo, PC, Desvarieux, M, Papapanou, PN, Jacobs, DR, Knight, R, Demmer, RT (2022-05-17). Nitrite Generating and Depleting Capacity of the Oral Microbiome and Cardiometabolic Risk: Results from ORIGINS. Journal of the American Heart Association 11 (10) : e023038-. ScholarBank@NUS Repository. https://doi.org/10.1161/JAHA.121.023038
dc.identifier.issn20479980
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/230957
dc.description.abstractBACKGROUND: The enterosalivary nitrate– nitrite– nitric oxide (NO3 – NO2 – NO) pathway generates NO following oral microbiota-mediated production of salivary nitrite, potentially linking the oral microbiota to reduced cardiometabolic risk. Nitrite depletion by oral bacteria may also be important for determining the net nitrite available systemically. We examine if higher abundance of oral microbial genes favoring increased oral nitrite generation and decreased nitrite depletion is associated with a better cardiometabolic profile cross-sectionally. METHODS AND RESULTS: This study includes 764 adults (mean [SD] age 32 [9] years, 71% women) enrolled in ORIGINS (Oral Infections, Glucose Intolerance, and Insulin Resistance Study). Microbial DNA from subgingival dental plaques underwent 16S rRNA gene sequencing; PICRUSt2 was used to estimate functional gene profiles. To represent the different components and pathways of nitrogen metabolism in bacteria, predicted gene abundances were operationalized to create summary scores by (1) bacterial nitrogen metabolic pathway or (2) biochemical product (NO2, NO, or ammonia [NH3 ]) formed by the action of the bacterial reductases encoded. Finally, nitrite generation-to-depletion ratios of gene abundances were created from the above summary scores. A composite cardiometabolic Z score was created from cardiometabolic risk variables, with higher scores associated with worse cardiometabolic health. We performed multivariable linear regression analysis with cardiometabolic Z score as the outcome and the gene abundance summary scores and ratios as predictor variables, adjusting for sex, age, race, and ethnicity in the simple adjusted model. A 1 SD higher NO versus NH3 summary ratio was inversely associated with a −0.10 (false discovery rate q=0.003) lower composite cardiometabolic Z score in simple adjusted models. Higher NH3 summary score (suggestive of nitrite depletion) was associated with higher cardiometabolic risk, with a 0.06 (false discovery rate q=0.04) higher composite cardiometabolic Z score. CONCLUSIONS: Increased net capacity for nitrite generation versus depletion by oral bacteria, assessed through a metagenome estimation approach, is associated with lower levels of cardiometabolic risk.
dc.publisherOvid Technologies (Wolters Kluwer Health)
dc.sourceElements
dc.subject16S rNA sequencing
dc.subjectblood pressure
dc.subjectepidemiology
dc.subjectinsulin resistance
dc.subjectmetagenomics
dc.subjectnitric oxide
dc.subjectoral microbiome
dc.subjectAdult
dc.subjectBacteria
dc.subjectCardiovascular Diseases
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectMicrobiota
dc.subjectNitrates
dc.subjectNitric Oxide
dc.subjectNitrites
dc.subjectNitrogen
dc.subjectNitrogen Dioxide
dc.subjectRNA, Ribosomal, 16S
dc.typeArticle
dc.date.updated2022-09-08T07:43:32Z
dc.contributor.departmentDEPT OF DENTISTRY
dc.description.doi10.1161/JAHA.121.023038
dc.description.sourcetitleJournal of the American Heart Association
dc.description.volume11
dc.description.issue10
dc.description.pagee023038-
dc.published.statePublished
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