Please use this identifier to cite or link to this item: https://doi.org/10.1161/JAHA.121.023038
Title: Nitrite Generating and Depleting Capacity of the Oral Microbiome and Cardiometabolic Risk: Results from ORIGINS
Authors: Goh, CE 
Bohn, B
Marotz, C
Molinsky, R
Roy, S
Paster, BJ
Chen, CY
Rosenbaum, M
Yuzefpolskaya, M
Colombo, PC
Desvarieux, M
Papapanou, PN
Jacobs, DR
Knight, R
Demmer, RT
Keywords: 16S rNA sequencing
blood pressure
epidemiology
insulin resistance
metagenomics
nitric oxide
oral microbiome
Adult
Bacteria
Cardiovascular Diseases
Female
Humans
Male
Microbiota
Nitrates
Nitric Oxide
Nitrites
Nitrogen
Nitrogen Dioxide
RNA, Ribosomal, 16S
Issue Date: 17-May-2022
Publisher: Ovid Technologies (Wolters Kluwer Health)
Citation: Goh, CE, Bohn, B, Marotz, C, Molinsky, R, Roy, S, Paster, BJ, Chen, CY, Rosenbaum, M, Yuzefpolskaya, M, Colombo, PC, Desvarieux, M, Papapanou, PN, Jacobs, DR, Knight, R, Demmer, RT (2022-05-17). Nitrite Generating and Depleting Capacity of the Oral Microbiome and Cardiometabolic Risk: Results from ORIGINS. Journal of the American Heart Association 11 (10) : e023038-. ScholarBank@NUS Repository. https://doi.org/10.1161/JAHA.121.023038
Abstract: BACKGROUND: The enterosalivary nitrate– nitrite– nitric oxide (NO3 – NO2 – NO) pathway generates NO following oral microbiota-mediated production of salivary nitrite, potentially linking the oral microbiota to reduced cardiometabolic risk. Nitrite depletion by oral bacteria may also be important for determining the net nitrite available systemically. We examine if higher abundance of oral microbial genes favoring increased oral nitrite generation and decreased nitrite depletion is associated with a better cardiometabolic profile cross-sectionally. METHODS AND RESULTS: This study includes 764 adults (mean [SD] age 32 [9] years, 71% women) enrolled in ORIGINS (Oral Infections, Glucose Intolerance, and Insulin Resistance Study). Microbial DNA from subgingival dental plaques underwent 16S rRNA gene sequencing; PICRUSt2 was used to estimate functional gene profiles. To represent the different components and pathways of nitrogen metabolism in bacteria, predicted gene abundances were operationalized to create summary scores by (1) bacterial nitrogen metabolic pathway or (2) biochemical product (NO2, NO, or ammonia [NH3 ]) formed by the action of the bacterial reductases encoded. Finally, nitrite generation-to-depletion ratios of gene abundances were created from the above summary scores. A composite cardiometabolic Z score was created from cardiometabolic risk variables, with higher scores associated with worse cardiometabolic health. We performed multivariable linear regression analysis with cardiometabolic Z score as the outcome and the gene abundance summary scores and ratios as predictor variables, adjusting for sex, age, race, and ethnicity in the simple adjusted model. A 1 SD higher NO versus NH3 summary ratio was inversely associated with a −0.10 (false discovery rate q=0.003) lower composite cardiometabolic Z score in simple adjusted models. Higher NH3 summary score (suggestive of nitrite depletion) was associated with higher cardiometabolic risk, with a 0.06 (false discovery rate q=0.04) higher composite cardiometabolic Z score. CONCLUSIONS: Increased net capacity for nitrite generation versus depletion by oral bacteria, assessed through a metagenome estimation approach, is associated with lower levels of cardiometabolic risk.
Source Title: Journal of the American Heart Association
URI: https://scholarbank.nus.edu.sg/handle/10635/230957
ISSN: 20479980
DOI: 10.1161/JAHA.121.023038
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
JAHA.121.023038.pdfPublished version2.23 MBAdobe PDF

OPEN

PublishedView/Download

Page view(s)

12
checked on Sep 15, 2022

Download(s)

1
checked on Sep 15, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.