Please use this identifier to cite or link to this item:
https://doi.org/10.2147/JIR.S369385Journalof
DC Field | Value | |
---|---|---|
dc.title | Moderate Dose Irradiation Induces DNA Damage and Impairments of Barrier and Host Defense in Nasal Epithelial Cells in vitro | |
dc.contributor.author | Yang, Yue-Ying | |
dc.contributor.author | Liu, Jing | |
dc.contributor.author | Liu, Yi-Tong | |
dc.contributor.author | Ong, Hsiao-Hui | |
dc.contributor.author | Chen, Qian-Min | |
dc.contributor.author | Chen, Ce-Belle | |
dc.contributor.author | Thong, Mark | |
dc.contributor.author | Xu, Xinni | |
dc.contributor.author | Zhou, Sui-Zi | |
dc.contributor.author | Qiu, Qian-Hui | |
dc.contributor.author | Wang, De-Yun | |
dc.date.accessioned | 2022-09-01T04:16:48Z | |
dc.date.available | 2022-09-01T04:16:48Z | |
dc.date.issued | 2022-01-01 | |
dc.identifier.citation | Yang, Yue-Ying, Liu, Jing, Liu, Yi-Tong, Ong, Hsiao-Hui, Chen, Qian-Min, Chen, Ce-Belle, Thong, Mark, Xu, Xinni, Zhou, Sui-Zi, Qiu, Qian-Hui, Wang, De-Yun (2022-01-01). Moderate Dose Irradiation Induces DNA Damage and Impairments of Barrier and Host Defense in Nasal Epithelial Cells in vitro. JOURNAL OF INFLAMMATION RESEARCH 15 : 3661-3675. ScholarBank@NUS Repository. https://doi.org/10.2147/JIR.S369385Journalof | |
dc.identifier.issn | 1178-7031 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/230739 | |
dc.description.abstract | Purpose: Radiotherapy (RT) is the mainstay treatment for head and neck cancers. However, chronic and recurrent upper respiratory tract infections and inflammation have been commonly reported in patients post-RT. The underlying mechanisms remain poorly understood. Method and Materials: We used a well-established model of human nasal epithelial cells (hNECs) that forms a pseudostratified layer in the air-liquid interface (ALI) and exposed it to single or repeated moderate dose γ-irradiation (1Gy). We assessed the DNA damage and evaluated the biological properties of hNECs at different time points post-RT. Further, we explored the host immunity alterations in irradiated hNECs with polyinosinic-polycytidylic acid sodium salt (poly [I:C]) and lipopolysaccharides (LPS). Results: IR induced DNA double strand breaks (DSBs) and triggered DNA damage response in hNECs. Repeated IR significantly reduced basal cell proliferation with low expression of p63/KRT5 and Ki67, induced cilia loss and inhibited mucus secretion. In addition, IR decreased ZO-1 expression and caused a significant decline in the transepithelial electrical resistance (TEER). Moreover, hyperreac-tive response against pathogen invasion and disrupted epithelial host defense can be observed in hNECs exposed to repeated IR. Conclusion: Our study suggests that IR induced prolonged structural and functional impairments of hNECs may contribute to patients post-RT with increased risk of developing chronic and recurrent upper respiratory tract infection and inflammation. | |
dc.language.iso | en | |
dc.publisher | DOVE MEDICAL PRESS LTD | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Immunology | |
dc.subject | human nasal epithelial cells | |
dc.subject | irradiation | |
dc.subject | DNA double strand breaks | |
dc.subject | epithelial barrier | |
dc.subject | host defense | |
dc.subject | DOUBLE-STRAND BREAKS | |
dc.subject | NASOPHARYNGEAL CARCINOMA | |
dc.subject | MUCOCILIARY CLEARANCE | |
dc.subject | RADIOTHERAPY | |
dc.subject | GAMMA-H2AX | |
dc.subject | RECRUITMENT | |
dc.subject | THERAPY | |
dc.subject | REPAIR | |
dc.type | Article | |
dc.date.updated | 2022-09-01T00:35:27Z | |
dc.contributor.department | OTOLARYNGOLOGY | |
dc.contributor.department | PHYSICS | |
dc.description.doi | 10.2147/JIR.S369385Journalof | |
dc.description.sourcetitle | JOURNAL OF INFLAMMATION RESEARCH | |
dc.description.volume | 15 | |
dc.description.page | 3661-3675 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Moderate Dose Irradiation Induces DNA Damage and Impairments of Barrier and Host Defense in Nasal Epithelial Cells in vitro.pdf | 9.27 MB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.