Please use this identifier to cite or link to this item: https://doi.org/10.2147/JIR.S369385Journalof
Title: Moderate Dose Irradiation Induces DNA Damage and Impairments of Barrier and Host Defense in Nasal Epithelial Cells in vitro
Authors: Yang, Yue-Ying
Liu, Jing 
Liu, Yi-Tong
Ong, Hsiao-Hui 
Chen, Qian-Min
Chen, Ce-Belle 
Thong, Mark 
Xu, Xinni 
Zhou, Sui-Zi
Qiu, Qian-Hui
Wang, De-Yun 
Keywords: Science & Technology
Life Sciences & Biomedicine
Immunology
human nasal epithelial cells
irradiation
DNA double strand breaks
epithelial barrier
host defense
DOUBLE-STRAND BREAKS
NASOPHARYNGEAL CARCINOMA
MUCOCILIARY CLEARANCE
RADIOTHERAPY
GAMMA-H2AX
RECRUITMENT
THERAPY
REPAIR
Issue Date: 1-Jan-2022
Publisher: DOVE MEDICAL PRESS LTD
Citation: Yang, Yue-Ying, Liu, Jing, Liu, Yi-Tong, Ong, Hsiao-Hui, Chen, Qian-Min, Chen, Ce-Belle, Thong, Mark, Xu, Xinni, Zhou, Sui-Zi, Qiu, Qian-Hui, Wang, De-Yun (2022-01-01). Moderate Dose Irradiation Induces DNA Damage and Impairments of Barrier and Host Defense in Nasal Epithelial Cells in vitro. JOURNAL OF INFLAMMATION RESEARCH 15 : 3661-3675. ScholarBank@NUS Repository. https://doi.org/10.2147/JIR.S369385Journalof
Abstract: Purpose: Radiotherapy (RT) is the mainstay treatment for head and neck cancers. However, chronic and recurrent upper respiratory tract infections and inflammation have been commonly reported in patients post-RT. The underlying mechanisms remain poorly understood. Method and Materials: We used a well-established model of human nasal epithelial cells (hNECs) that forms a pseudostratified layer in the air-liquid interface (ALI) and exposed it to single or repeated moderate dose γ-irradiation (1Gy). We assessed the DNA damage and evaluated the biological properties of hNECs at different time points post-RT. Further, we explored the host immunity alterations in irradiated hNECs with polyinosinic-polycytidylic acid sodium salt (poly [I:C]) and lipopolysaccharides (LPS). Results: IR induced DNA double strand breaks (DSBs) and triggered DNA damage response in hNECs. Repeated IR significantly reduced basal cell proliferation with low expression of p63/KRT5 and Ki67, induced cilia loss and inhibited mucus secretion. In addition, IR decreased ZO-1 expression and caused a significant decline in the transepithelial electrical resistance (TEER). Moreover, hyperreac-tive response against pathogen invasion and disrupted epithelial host defense can be observed in hNECs exposed to repeated IR. Conclusion: Our study suggests that IR induced prolonged structural and functional impairments of hNECs may contribute to patients post-RT with increased risk of developing chronic and recurrent upper respiratory tract infection and inflammation.
Source Title: JOURNAL OF INFLAMMATION RESEARCH
URI: https://scholarbank.nus.edu.sg/handle/10635/230739
ISSN: 1178-7031
DOI: 10.2147/JIR.S369385Journalof
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