CELLULAR RESOURCE MODELS FOR GENETIC CIRCUIT DESIGN

Abstract

Understanding cellular resource use is required for the predictive engineering of heterologous expression. In this study, the ribosome dynamics of E.coli were characterized under non-constant conditions and with/without heterologous GFP expression. It was observed that ribosome abundance and growth rate showed a cyclical relationship and that GFP expression did not depress ribosome abundance. A coarse-grain kinetic model was then built to recapitulate and explain the results.