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Title: Bio-functionalization of Electrospun Nanofibre Scaffolds for Cell Culture Applications
Keywords: Electrospinning, Nanofiber, Surface grafting polymerization, Ligand immobilization, Hepatocyte spheroid culture, Hematopoietic stem cell expansion
Issue Date: 5-Mar-2007
Citation: CHUA KIAN NGIAP (2007-03-05). Bio-functionalization of Electrospun Nanofibre Scaffolds for Cell Culture Applications. ScholarBank@NUS Repository.
Abstract: The objective of this thesis is to develop polymeric nanofiber constructs that can serve as cell culture scaffolds, which can promote cell-substrate interactions and are bioactive in soliciting favorable cellular responses like cell adhesion, morphological reorganization, differentiated functions or proliferation. The strategy of scaffold development involves nanofiber scaffold fabrication via the electrospinning technique, followed by nanofiber bio-functionalization. The process involves the initial functionalization of the nanofiber surface with carboxylic acid groups using UV-initiated poly(acrylic acid) grafting method. This is followed by conjugation of bioactive molecules onto the functionalized nanofiber surfaces. In this thesis, we will test the efficacy of this nanofiber bio-functionalization strategy on two primary cell culture systems: hepatocyte spheroid cultures and hematopoietic stem / progenitor cell expansion cultures. The effect of immobilized bioactive molecules in promoting cell-substrate interactions will be investigated. We will also be focusing on the effect of nanofiber topography in synergistically enhancing these cell-substrate interactions.
Appears in Collections:Ph.D Theses (Open)

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