Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2021.768868
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dc.titleGenomic Characterization of a Novel Freshwater Cyanophage Reveals a New Lineage of Cyanopodovirus
dc.contributor.authorZhang, D
dc.contributor.authorHe, Y
dc.contributor.authorGin, KYH
dc.date.accessioned2022-08-15T13:15:18Z
dc.date.available2022-08-15T13:15:18Z
dc.date.issued2022-01-12
dc.identifier.citationZhang, D, He, Y, Gin, KYH (2022-01-12). Genomic Characterization of a Novel Freshwater Cyanophage Reveals a New Lineage of Cyanopodovirus. Frontiers in Microbiology 12 : 768868-. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2021.768868
dc.identifier.issn1664-302X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/230303
dc.description.abstractCyanobacteria are one of the dominant autotrophs in tropical freshwater communities, yet phages infecting them remain poorly characterized. Here we present the characterization of cyanophage S-SRP02, isolated from a tropical freshwater lake in Singapore, which infects Synechococcus sp. Strain SR-C1 isolated from the same lake. S-SRP02 represents a new evolutionary lineage of cyanophage. Out of 47 open reading frames (ORFs), only 20 ORFs share homology with genes encoding proteins of known function. There is lack of auxiliary metabolic genes which was commonly found as core genes in marine cyanopodoviruses. S-SRP02 also harbors unique structural genes highly divergent from other cultured phages. Phylogenetic analysis and viral proteomic tree further demonstrate the divergence of S-SRP02 from other sequenced phage isolates. Nonetheless, S-SRP02 shares synteny with phage genes of uncultured phages obtained from the Mediterranean Sea deep chlorophyll maximum fosmids, indicating the ecological importance of S-SRP02 and its related viruses. This is further supported by metagenomic mapping of environmental viral metagenomic reads onto the S-SRP02 genome.
dc.publisherFrontiers Media SA
dc.sourceElements
dc.subjectcyanophage
dc.subjectfreshwater Synechococcus
dc.subjectmetagenomic mapping
dc.subjectnew evolutionary lineage
dc.subjectphylogenetic analysis
dc.subjecttropical freshwater environments
dc.typeArticle
dc.date.updated2022-08-13T02:20:26Z
dc.contributor.departmentCIVIL AND ENVIRONMENTAL ENGINEERING
dc.contributor.departmentNUS ENVIRONMENTAL RESEARCH INSTITUTE
dc.description.doi10.3389/fmicb.2021.768868
dc.description.sourcetitleFrontiers in Microbiology
dc.description.volume12
dc.description.page768868-
dc.published.statePublished
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