Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ygeno.2005.12.006
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dc.titleThe complexity of antisense transcription revealed by the study of developing male germ cells
dc.contributor.authorChan, Wai-Yee
dc.contributor.authorWu, Shao-Ming
dc.contributor.authorRuszczyk, Lisa
dc.contributor.authorLaw, Evelyn
dc.contributor.authorLee, Tin-Lap
dc.contributor.authorBaxendale, Vanessa
dc.contributor.authorPang, Alan Lap-Yin
dc.contributor.authorRennert, Owen M
dc.date.accessioned2022-07-20T02:02:12Z
dc.date.available2022-07-20T02:02:12Z
dc.date.issued2006-06-01
dc.identifier.citationChan, Wai-Yee, Wu, Shao-Ming, Ruszczyk, Lisa, Law, Evelyn, Lee, Tin-Lap, Baxendale, Vanessa, Pang, Alan Lap-Yin, Rennert, Owen M (2006-06-01). The complexity of antisense transcription revealed by the study of developing male germ cells. GENOMICS 87 (6) : 681-692. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ygeno.2005.12.006
dc.identifier.issn08887543
dc.identifier.issn10898646
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/228861
dc.description.abstractComputational analyses have identified the widespread occurrence of antisense transcripts in the human and the mouse genome. However, the structure and the origin of the majority of the antisense transcripts are unknown. The presence of antisense transcripts for 19 of 64 differentially expressed genes during mouse spermatogenesis was demonstrated with orientation-specific RT-PCR. These antisense transcripts were derived from a wide variety of origins, including processed sense transcripts, intronic and exonic sequences of a single gene or multiple genes, intergenic sequences, and pseudogenes. They underwent normal and alternative splicing, 5′ capping, and 3′ polyadenylation, similar to the sense transcripts. There were also antisense transcripts that were not capped and/or polyadenylated. The testicular levels of the sense transcripts were higher than those of the antisense transcripts in all cases, while the relative expression in nontesticular tissues was variable. Thus antisense transcripts have complex origins and structures and the sense and antisense transcripts can be regulated independently.
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiotechnology & Applied Microbiology
dc.subjectGenetics & Heredity
dc.subjectantisense transcription
dc.subjectintron
dc.subjectexon
dc.subjectintergenic
dc.subjectpseudogene
dc.subjectmouse
dc.subjectspermatogonia
dc.subjectspermatocytes
dc.subjectspermatids
dc.subjectNITRIC-OXIDE SYNTHASE
dc.subjectMESSENGER-RNA
dc.subjectPOSTTRANSCRIPTIONAL REGULATION
dc.subjectSPERMATOGENIC CELLS
dc.subjectHUMAN GENOME
dc.subjectIN-VIVO
dc.subjectEXPRESSION
dc.subjectGENES
dc.subjectSENSE
dc.subjectMOUSE
dc.typeArticle
dc.date.updated2022-07-16T01:22:03Z
dc.contributor.departmentDEPT OF PAEDIATRICS
dc.description.doi10.1016/j.ygeno.2005.12.006
dc.description.sourcetitleGENOMICS
dc.description.volume87
dc.description.issue6
dc.description.page681-692
dc.published.statePublished
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