Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ygeno.2005.12.006
Title: The complexity of antisense transcription revealed by the study of developing male germ cells
Authors: Chan, Wai-Yee
Wu, Shao-Ming
Ruszczyk, Lisa
Law, Evelyn 
Lee, Tin-Lap
Baxendale, Vanessa
Pang, Alan Lap-Yin
Rennert, Owen M
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
antisense transcription
intron
exon
intergenic
pseudogene
mouse
spermatogonia
spermatocytes
spermatids
NITRIC-OXIDE SYNTHASE
MESSENGER-RNA
POSTTRANSCRIPTIONAL REGULATION
SPERMATOGENIC CELLS
HUMAN GENOME
IN-VIVO
EXPRESSION
GENES
SENSE
MOUSE
Issue Date: 1-Jun-2006
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation: Chan, Wai-Yee, Wu, Shao-Ming, Ruszczyk, Lisa, Law, Evelyn, Lee, Tin-Lap, Baxendale, Vanessa, Pang, Alan Lap-Yin, Rennert, Owen M (2006-06-01). The complexity of antisense transcription revealed by the study of developing male germ cells. GENOMICS 87 (6) : 681-692. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ygeno.2005.12.006
Abstract: Computational analyses have identified the widespread occurrence of antisense transcripts in the human and the mouse genome. However, the structure and the origin of the majority of the antisense transcripts are unknown. The presence of antisense transcripts for 19 of 64 differentially expressed genes during mouse spermatogenesis was demonstrated with orientation-specific RT-PCR. These antisense transcripts were derived from a wide variety of origins, including processed sense transcripts, intronic and exonic sequences of a single gene or multiple genes, intergenic sequences, and pseudogenes. They underwent normal and alternative splicing, 5′ capping, and 3′ polyadenylation, similar to the sense transcripts. There were also antisense transcripts that were not capped and/or polyadenylated. The testicular levels of the sense transcripts were higher than those of the antisense transcripts in all cases, while the relative expression in nontesticular tissues was variable. Thus antisense transcripts have complex origins and structures and the sense and antisense transcripts can be regulated independently.
Source Title: GENOMICS
URI: https://scholarbank.nus.edu.sg/handle/10635/228861
ISSN: 08887543
10898646
DOI: 10.1016/j.ygeno.2005.12.006
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