Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.molonc.2016.05.006
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dc.titleGlobal re-wiring of p53 transcription regulation by the hepatitis B virus X protein
dc.contributor.authorChan, Cheryl
dc.contributor.authorThurnherr, Thomas
dc.contributor.authorWang, Jingbo
dc.contributor.authorGallart-Palau, Xavier
dc.contributor.authorSze, Siu Kwan
dc.contributor.authorRozen, Steve
dc.contributor.authorLee, Caroline G
dc.date.accessioned2022-06-09T13:45:54Z
dc.date.available2022-06-09T13:45:54Z
dc.date.issued2016-10-01
dc.identifier.citationChan, Cheryl, Thurnherr, Thomas, Wang, Jingbo, Gallart-Palau, Xavier, Sze, Siu Kwan, Rozen, Steve, Lee, Caroline G (2016-10-01). Global re-wiring of p53 transcription regulation by the hepatitis B virus X protein. MOLECULAR ONCOLOGY 10 (8) : 1183-1195. ScholarBank@NUS Repository. https://doi.org/10.1016/j.molonc.2016.05.006
dc.identifier.issn1878-0261
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/226882
dc.description.abstractBackground The tumour suppressor p53 is a central player in transcription regulation and cell fate determination. By interacting with p53 and altering its sequence-specific binding to the response elements, the hepatitis B virus X protein (HBx) was reported to re-direct p53 regulation of some genes. Results Coupling massively parallel deep sequencing with p53 chromatin immunoprecipitation, we demonstrate that HBx modulates global p53 site selection and that this was strongly influenced by altered interaction with transcription co-factors/co-regulators as well as post-translational modifications. Specifically, HBx attenuated p53-TBP-RB1 transcription complex recruitment and interaction and this was associated with hyper-phosphorylation of p53 at serine 315 by HBx. Concurrently, HBx enhanced p53 DNA occupancy to other response elements either alone by displacing specific transcription factors such as CEBPB and NFkB1, or in complex with distinct interacting co-factors Sp1, JUN and E2F1. Importantly, re-wiring of p53 transcription regulation by HBx was linked to the deregulation of genes involved in cell proliferation and death, suggesting a role of HBx in errant cell fate determination mediated by altered p53 site selection of target genes. Conclusions Our study thus presents first evidence of global modes of p53 transcription alteration by HBx and provides new insights to understand and potentially curtail the viral oncoprotein.
dc.language.isoen
dc.publisherWILEY
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectOncology
dc.subjectP53 transcription
dc.subjectHBx
dc.subjectChIP-Seq
dc.subjectPhosphorylation
dc.subjectp53 serine 315
dc.subjectCELL-CYCLE ARREST
dc.subjectDNA-BINDING
dc.subjectGENE-EXPRESSION
dc.subjectIN-VIVO
dc.subjectGENOME
dc.subjectSITES
dc.subjectTRANSACTIVATION
dc.subjectACETYLATION
dc.subjectASSOCIATION
dc.subjectINHIBITION
dc.typeArticle
dc.date.updated2022-06-07T07:02:23Z
dc.contributor.departmentBIOLOGY (NU)
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.contributor.departmentNUS GRADUATE SCHOOL
dc.description.doi10.1016/j.molonc.2016.05.006
dc.description.sourcetitleMOLECULAR ONCOLOGY
dc.description.volume10
dc.description.issue8
dc.description.page1183-1195
dc.published.statePublished
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