Please use this identifier to cite or link to this item: https://doi.org/10.1117/1.JBO.22.5.050502
Title: Line-scan focal modulation microscopy
Authors: Pant, Shilpa 
Li, Caixia 
Gong, Zhiyuan 
Chen, Nanguang 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Optics
Radiology, Nuclear Medicine & Medical Imaging
Biochemistry & Molecular Biology
fluorescence microscopy
confocal
line-scan
focal modulation
speed
background rejection
PLANE ILLUMINATION MICROSCOPY
THICK BIOLOGICAL TISSUES
FLUORESCENCE MICROSCOPY
OPTICAL-PROPERTIES
ZEBRAFISH
CELLS
Issue Date: 1-May-2017
Publisher: SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Citation: Pant, Shilpa, Li, Caixia, Gong, Zhiyuan, Chen, Nanguang (2017-05-01). Line-scan focal modulation microscopy. JOURNAL OF BIOMEDICAL OPTICS 22 (5). ScholarBank@NUS Repository. https://doi.org/10.1117/1.JBO.22.5.050502
Abstract: We report the development of a line-scan focal modulation microscope (LSFMM) that is capable of high-speed image acquisition (>40 fps) with uncompromised optical sectioning capability. The improved background rejection and axial resolution of this imaging modality, enabled by focal modulation, are quantified with three-dimensional imaging data obtained from fluorescent beads. The signal-to-background ratio for the LSFMM system is one-to two-orders of magnitude higher than that for line-scanning confocal systems when imaging deep (up to 100 μm) into a turbid medium of optical properties similar to biological tissues. The imaging performance of LSFMM, in terms of both spatial and temporal resolutions, is further demonstrated with in vivo imaging experiments with live zebrafish larvae.
Source Title: JOURNAL OF BIOMEDICAL OPTICS
URI: https://scholarbank.nus.edu.sg/handle/10635/226843
ISSN: 1083-3668,1560-2281
DOI: 10.1117/1.JBO.22.5.050502
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