Please use this identifier to cite or link to this item: https://doi.org/10.1364/BOE.8.005698
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dc.titleAugmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart &ITin vivo&IT
dc.contributor.authorPant, Shilpa
dc.contributor.authorDuan, Yubo
dc.contributor.authorXiong, Fei
dc.contributor.authorChen, Nanguang
dc.date.accessioned2022-06-09T04:23:15Z
dc.date.available2022-06-09T04:23:15Z
dc.date.issued2017-12-01
dc.identifier.citationPant, Shilpa, Duan, Yubo, Xiong, Fei, Chen, Nanguang (2017-12-01). Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart &ITin vivo&IT. BIOMEDICAL OPTICS EXPRESS 8 (12) : 5698-5707. ScholarBank@NUS Repository. https://doi.org/10.1364/BOE.8.005698
dc.identifier.issn2156-7085,2156-7085
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/226842
dc.description.abstractMulti-dimensional fluorescence imaging of live animal models demands strong optical sectioning, high spatial resolution, fast image acquisition, and minimal photobleaching. While conventional laser scanning microscopes are capable of deep penetration and sub-cellular resolution, they are generally too slow and causing excessive photobleaching for volumetric or time-lapse imaging. We demonstrate the performance of an augmented line-scan focal modulation microscope (aLSFMM), a high-speed imaging platform that affords above video-rate imaging speed by the use of line scanning. Exceptional background rejection is accomplished by combining a confocal slit with focal modulation. The image quality is further improved by merging the information from simultaneously acquired focal modulation and confocal images. Such a hybrid imaging scheme makes it possible to use very low power excitation light in high-speed imaging, and therefore leads to reduced photobleaching that is desirable for three-dimensional (3D) and four-dimensional (4D) in vivo image acquisition.
dc.language.isoen
dc.publisherOPTICAL SOC AMER
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysical Sciences
dc.subjectBiochemical Research Methods
dc.subjectOptics
dc.subjectRadiology, Nuclear Medicine & Medical Imaging
dc.subjectBiochemistry & Molecular Biology
dc.subjectLIGHT-SHEET MICROSCOPY
dc.subjectILLUMINATION MICROSCOPY
dc.subjectFLUORESCENCE MICROSCOPY
dc.subjectRECONSTRUCTION
dc.subjectEMBRYOS
dc.subjectDEEP
dc.typeArticle
dc.date.updated2022-06-07T03:57:03Z
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentBIOMEDICAL ENGINEERING
dc.contributor.departmentDEPT OF BIOMEDICAL ENGINEERING
dc.description.doi10.1364/BOE.8.005698
dc.description.sourcetitleBIOMEDICAL OPTICS EXPRESS
dc.description.volume8
dc.description.issue12
dc.description.page5698-5707
dc.published.statePublished
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