Please use this identifier to cite or link to this item: https://doi.org/10.1364/BOE.8.005698
Title: Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart &ITin vivo&IT
Authors: Pant, Shilpa 
Duan, Yubo 
Xiong, Fei 
Chen, Nanguang 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Optics
Radiology, Nuclear Medicine & Medical Imaging
Biochemistry & Molecular Biology
LIGHT-SHEET MICROSCOPY
ILLUMINATION MICROSCOPY
FLUORESCENCE MICROSCOPY
RECONSTRUCTION
EMBRYOS
DEEP
Issue Date: 1-Dec-2017
Publisher: OPTICAL SOC AMER
Citation: Pant, Shilpa, Duan, Yubo, Xiong, Fei, Chen, Nanguang (2017-12-01). Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart &ITin vivo&IT. BIOMEDICAL OPTICS EXPRESS 8 (12) : 5698-5707. ScholarBank@NUS Repository. https://doi.org/10.1364/BOE.8.005698
Abstract: Multi-dimensional fluorescence imaging of live animal models demands strong optical sectioning, high spatial resolution, fast image acquisition, and minimal photobleaching. While conventional laser scanning microscopes are capable of deep penetration and sub-cellular resolution, they are generally too slow and causing excessive photobleaching for volumetric or time-lapse imaging. We demonstrate the performance of an augmented line-scan focal modulation microscope (aLSFMM), a high-speed imaging platform that affords above video-rate imaging speed by the use of line scanning. Exceptional background rejection is accomplished by combining a confocal slit with focal modulation. The image quality is further improved by merging the information from simultaneously acquired focal modulation and confocal images. Such a hybrid imaging scheme makes it possible to use very low power excitation light in high-speed imaging, and therefore leads to reduced photobleaching that is desirable for three-dimensional (3D) and four-dimensional (4D) in vivo image acquisition.
Source Title: BIOMEDICAL OPTICS EXPRESS
URI: https://scholarbank.nus.edu.sg/handle/10635/226842
ISSN: 2156-7085,2156-7085
DOI: 10.1364/BOE.8.005698
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