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|Title:||UT HOURS COMPLETED FOR AY06/07 SEM 1 = 48HRS UT HOURS COMPLETED FOR AY06/07 SEM 2 = 16HRS||Authors:||GAN HUI THENG||Keywords:||neural stem cells, Apolipoprotein E, ERK signaling, oligodendrocytes, Sox10, GABARAP||Issue Date:||30-Apr-2010||Citation:||GAN HUI THENG (2010-04-30). UT HOURS COMPLETED FOR AY06/07 SEM 1 = 48HRS UT HOURS COMPLETED FOR AY06/07 SEM 2 = 16HRS. ScholarBank@NUS Repository.||Abstract:||Neural stem cells (NSCs) are the self-renewing and multipotent cells that reside in the subventricular zone of the lateral ventricle, and subgranular zone of the adult hippocampus. At the embryonic stage, they are the neuroepithelial cells lining the ventricular zone. These cells are potentially useful for cell replacement therapies, drug delivery, drug development, and as a tool for understanding neurological diseases. NSCs are regulated by the microenvironment that provides information for its maintenance or differentiation. ApoE has been identified as one of the key components of NSC conditioned-medium1. This thesis describes the role of ApoE in promoting neurosphere formation through the activation of Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase (MAPK/ERK) signaling pathway. Besides stimulating neurosphere formation from primary NSCs/NPs, ApoE also stimulated neurosphere formation of ESC-derived NSCs/NPs. Using a combination of clonal density culture and single neurosphere differentiation, ApoE was demonstrated to stimulate neurosphere formation by enhancing NSC survival. In addition, ApoE is also involved in differentiation of NSCs/NPs, specifically oligodendrogenesis. Besides the NSC microenvironment, NSCs are also regulated by transcriptional control. Sox10 transcription factor is essential in the development of oligodendrocytes. Here, work was done in identifying the interaction of Sex determining Region Y-box 10 (Sox10), a transcription factor pertinent to oligodendrogenesis of NSCs, and Gamma- AminoButyric Acid Receptor-Associated Protein (GABARAP). This interaction may be important for regulating Sox10-induction of oligodendrogenesis. Overall, this thesis describes the regulation of NSC proliferation and differentiation by extracellular and intracellular proteins.||URI:||http://scholarbank.nus.edu.sg/handle/10635/22683|
|Appears in Collections:||Ph.D Theses (Open)|
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