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Title: Functional studies of the Y-box binding protein 1 in Breast cancer
Keywords: Y-box binding protein 1, breast cancer, proliferation, epithelial-mesenchymal transition, metastasis
Issue Date: 20-Aug-2010
Citation: YU YINGNAN (2010-08-20). Functional studies of the Y-box binding protein 1 in Breast cancer. ScholarBank@NUS Repository.
Abstract: Breast cancer is the most prevalent malignancy among women worldwide. It is imperative to identify clinically useful biomarkers for better treatment and management of this disease. The Y-box-binding protein 1 (YB-1), a DNA- and RNA- binding protein, has pleiotropic functions and was found to implicate cancer progression. In this study, YB-1 expression in breast cancer tissue microarrays was found to be positively correlated with axillary lymph node spread, mitotic index and Proliferating Cell Nuclear Antigen (PCNA) immunostaining, an established marker of cell proliferation. YB-1 gene expression level was also increased in advanced stages of breast cancer. In vitro experiments showed that downregulation of YB-1 inhibited cell proliferation and induced G1 phase cell cycle arrest. In vitro experiments also demonstrated that YB-1 enhanced breast cancer cell motility and invasion through induction of epithelial-mesenchymal transition. Genome-wide expression analysis by microarray showed that 109 genes were differentially regulated after downregulation of YB-1. The regulation of the proteins encoded by these genes could contribute to the observed breast cancer cell phenotypic alternations. The findings in this study provide evidence that YB-1 is possibly a potential molecular target in breast cancer therapy.
Appears in Collections:Ph.D Theses (Open)

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