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Title: Molecular Transport and Structure of DNA in A Congested State.
Keywords: DNA,transport,structure,congested state,viscoelasticity,neutron scattering
Issue Date: 28-Jul-2010
Citation: ZHU XIAOYING (2010-07-28). Molecular Transport and Structure of DNA in A Congested State.. ScholarBank@NUS Repository.
Abstract: In this thesis, the molecular transportation properties and the structure of supercoiled DNA is investigated. To study dynamic properties of DNA, the viscoelastic moduli of lambda phage DNA through the entanglement transition were obtained with particle tracking microrheology. The number of entanglements per chain is obtained. The longest, global relaxation time pertaining to the motion of the DNA molecules is obtained as well. A comprehensive characterization of viscoelasticity of DNA solutions with increasing concentration in terms of viscous loss and elastic storage moduli is explored. With a view to determining the distance between the two opposing duplexes in supercoiled DNA, small angle neutron scattering from pHSG298 plasmid dispersed in saline solutions were measured. Experiments were carried out under full and zero average DNA neutron scattering contrast for the first time. It was observed that the interduplex distance decreases with increasing concentration of salt as well as plasmid. Therefore, besides ionic strength, DNA crowding is shown to be important in controlling the interwound structure and site juxtaposition of distal segments of supercoiled DNA.
Appears in Collections:Ph.D Theses (Open)

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