Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12017-019-08586-y
Title: Ultrastructural Characteristics of DHA-Induced Pyroptosis
Authors: Herr, Deron R 
Yam, Ting Yu Amelia 
Tan, Wan Shun Daniel 
Koh, Sally Shuxian
Wong, Wai Shiu Fred 
Ong, Wei-Yi 
Chayaburakul, Kanokporn
Keywords: Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
Docosahexaenoic acid (DHA)
Pyroptosis
Scanning electron microscopy (SEM)
Transmission electron microscopy (TEM)
Membrane pores
Microglia
PROGRAMMED CELL-DEATH
GASDERMIN-D
INFLAMMATORY CASPASES
PORE FORMATION
APOPTOSIS
MECHANISM
BRAIN
GSDMD
SALMONELLA
ACTIVATION
Issue Date: 4-Jan-2020
Publisher: HUMANA PRESS INC
Citation: Herr, Deron R, Yam, Ting Yu Amelia, Tan, Wan Shun Daniel, Koh, Sally Shuxian, Wong, Wai Shiu Fred, Ong, Wei-Yi, Chayaburakul, Kanokporn (2020-01-04). Ultrastructural Characteristics of DHA-Induced Pyroptosis. NEUROMOLECULAR MEDICINE 22 (2) : 293-303. ScholarBank@NUS Repository. https://doi.org/10.1007/s12017-019-08586-y
Abstract: Microglial cells are resident macrophages of the central nervous system (CNS) that respond to bioactive lipids such as docosahexaenoic acid (DHA). Low micromolar concentrations of DHA typically promote anti-inflammatory functions of microglia, but higher concentrations result in a form of pro-inflammatory programmed cell death known as pyroptosis. This study used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to investigate the morphological characteristics of pyroptosis in BV-2 microglial cells following exposure to 200 µM DHA. Vehicle-treated cells are characterized by extended processes, spine-like projections or 0.4 to 5.2 µm in length, and numerous extracellular vesicles (EVs) tethered to the surface of the plasma membrane. In contrast to vehicle-treated cells, gross abnormalities are observed after treating cells with 200 µM DHA for 4 h. These include the appearance of numerous pits or pores of varying sizes across the cell surface, structural collapse and flattening of the cell shape. Moreover, EVs and spines were lost following DHA treatment, possibly due to release from the cell surface. The membrane pores appear after DHA treatment initially measured ~ 30 nm, consistent with the previously reported gasdermin D (GSDMD) pore complexes. Complete collapse of cytoplasmic organization and loss of nuclear envelope integrity were also observed in DHA-treated cells. These processes are morphologically distinct from the changes that occur during cisplatin-induced apoptosis, such as the appearance of apoptotic bodies and tightly packed organelles, and the maintenance of EVs and nuclear envelope integrity. Cumulatively, this study provides a systematic description of the ultrastructural characteristics of DHA-induced pyroptosis, including distinguishing features that differentiate this process from apoptosis.
Source Title: NEUROMOLECULAR MEDICINE
URI: https://scholarbank.nus.edu.sg/handle/10635/219306
ISSN: 15351084
15591174
DOI: 10.1007/s12017-019-08586-y
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