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Title: Genetically Encodable Scaffolds for Optimizing Enzyme Function
Authors: Tan, Yong Quan 
Xue, Bo 
Yew, Wen Shan 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
protein shells
synthetic enzymology
synthetic biology
protein scaffold
nucleic acid scaffold
Issue Date: 1-Mar-2021
Publisher: MDPI
Citation: Tan, Yong Quan, Xue, Bo, Yew, Wen Shan (2021-03-01). Genetically Encodable Scaffolds for Optimizing Enzyme Function. MOLECULES 26 (5). ScholarBank@NUS Repository.
Abstract: Enzyme engineering is an indispensable tool in the field of synthetic biology, where enzymes are challenged to carry out novel or improved functions. Achieving these goals sometimes goes beyond modifying the primary sequence of the enzyme itself. The use of protein or nucleic acid scaffolds to enhance enzyme properties has been reported for applications such as microbial production of chemicals, biosensor development and bioremediation. Key advantages of using these assemblies include optimizing reaction conditions, improving metabolic flux and increasing enzyme stability. This review summarizes recent trends in utilizing genetically encodable scaffolds, developed in line with synthetic biology methodologies, to complement the purposeful deployment of enzymes. Current molecular tools for constructing these synthetic enzyme-scaffold systems are also highlighted.
Source Title: MOLECULES
ISSN: 14203049
DOI: 10.3390/molecules26051389
Appears in Collections:Staff Publications

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